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J Herbmed Pharmacol. 2023;12(4): 512-520.
doi: 10.34172/jhp.2023.45002
  Abstract View: 636
  PDF Download: 263

Original Article

Anti-cancer effects of the extracts of broad and spirale cultivars of Codiaeum variegatum (L.) Blume on MCF-7, HepG2, and HeLa cell lines

Phanida Suphiratwanich 1 ORCID logo, Benjaporn Buranrat 2,3 ORCID logo, Supavadee Boontha 3,4* ORCID logo

1 Faculty of Pharmacy, Payap University, Chiang Mai, 51000, Thailand
2 Faculty of Medicine, Mahasarakham University, Maha Sarakham, 44000, Thailand
3 Research Group in Herbal and Development of Formulation and Delivery Systems for Elderly Adults and Cancer Treatment, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, Thailand
4 School of Pharmaceutical Sciences, University of Phayao, Phayao, 56000, Thailand
*Corresponding Author: Supavadee Boontha, Email: supavadee.bo@up.ac.th

Abstract

Introduction: Codiaeum variegatum (L.) Blume is a well-known ornamental foliage plant used as a vegetable in northern Thailand, and it is the source of numerous bioactive substances. This work explored the effects of leaf extracts of broad (BCE) and spirale (SCE) cultivars of C. variegatum on three cancer cells, including human breast, human liver, and human cervical cancer cells.

Methods: Ethanolic plant extracts were prepared, and then, 2,2-diphenyl-1-picryhydrazyl (DPPH), ferrous iron chelating, and lipid peroxidation assays were used to examine the flavonoid and phenolic compounds. The proliferative inhibition, growth, and migration of MCF-7, HepG2, and HeLa cancer cells, as a result of exposure to the extracts, were investigated. The extracts were investigated for their anti-cancer activities using sulforhodamine B (SRB), clonogenic, and wound-healing methods.

Results: The data demonstrated that BCE and SCE contained high phenolic compounds. However, both extracts showed inactive anti-oxidant activities. Both extracts had high cytotoxicity on three types of cancer cells in a dose- and time-dependent manner after 24-72 hours of incubation with IC50 values in a range of 208-830 µg/mL. Moreover, the prepared extracts of C. variegatum significantly inhibited colony-forming ability and cell migration on all types of cancer cells. Compared with BCE, SCE showed more potent anti-cancer activities.

Conclusion: These findings revealed that SCE had higher anti-cancer activities on MCF-7, HepG2, and HeLa cancer cells than BCE. Consequently, the SCE might be used as an effective chemotherapeutic compound for the prevention and treatment of cancer.


Implication for health policy/practice/research/medical education:

This study provides novel insights into the anti-cancer effects of extracts prepared from the leaves of broad and spirale cultivars of C. variegatum on breast (MCF-7), hepatoma (HepG2), and cervical (HeLa) cancer cell lines and their effective uses in treating hepatoma, breast, and cervical cancers.

Please cite this paper as: Suphiratwanich P, Buranrat B, Boontha S. Anti-cancer effects of the extracts of broad and spirale cultivars of Codiaeum variegatum (L.) Blume on MCF-7, HepG2, and HeLa cell lines. J Herbmed Pharmacol. 2023;12(4):512-520. doi: 10.34172/jhp.2023.45002.

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Submitted: 14 Apr 2023
Revision: 21 Jun 2023
Accepted: 29 Jun 2023
ePublished: 10 Aug 2023
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