J Herbmed Pharmacol. 2023;12(1): 73-83.
doi: 10.34172/jhp.2023.06

Scopus ID: 85147766540
  Abstract View: 1053
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Original Article

Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis

Jinjoo Kang 1 ORCID logo, YoungJae Kim 2 ORCID logo, Jae-Min Chung 3 ORCID logo, Namkyung Kim 1 ORCID logo, Young-Ae Choi 1 ORCID logo, Eun-Nam Kim 4 ORCID logo, Gil-Saeng Jeong 4* ORCID logo, Sang-Yong Kim 3* ORCID logo, Sang-Hyun Kim 1* ORCID logo

1 Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 41944, South Korea
2 Department of Gardens Education, Korea National Arboretum, Pocheon 11186, South Korea
3 Division of Plant Resources, Korea National Arboretum, Yangpyeong 12519, South Korea
4 College of Pharmacy, Chungnam National University, Daejeon 34134, South Korea
*Corresponding Author: Kyungpook National University Email gsjeong@kmu.ac.kr, rosaksy@korea.kr, shkim72@knu.ac.kr
*Corresponding Authors: Gil-Saeng Jeong, Email: gsjeong@kmu.ac.kr; Sang-Yong Kim, Email: rosaksy@korea.kr; Sang-Hyun Kim, Email: shkim72@knu.ac.kr


Introduction: Rhododendron brachycarpum D. Don ex G. Don(RbGD) leaves have traditionally been used to treat diabetes, rheumatoid arthritis, hypertension, and skin ailments. Although RbGD leaves are used as a medicinal plant, there is no scientific evidence to support skin treatment. Therefore, the purpose of this study was to look into the anti-inflammatory effect of an ethanolic extract of RbGD on skin disease, specifically atopic dermatitis (AD).

Methods: The anti-inflammatory effect of RbGD ethanol leaf extract (RbGDE) on tumor necrosis factor-α (TNF-α)/interferon-γ (IFN-γ)-activated keratinocytes was evaluated using MTT, qPCR, ELISA, and Western blot Procedures. The therapeutic effects of RbGDE were evaluated in vivo inflammatory responses by histological observation, quantitative polymerase chain reaction (qPCR), and ELISA using the 1-chloro-2,4-dinitrobenzene (DNCB)/Dermatophagoides farina extract (DfE)-induced AD-like skin mouse model.

Results: RbGDE showed the protective effect against irritating and stimulating substances (H2 O2 and TNF-α/IFN-γ) and inhibited TNF-α/IFN-γ-activated keratinocytes by inhibiting the p38 mitogen-activated protein kinase and nuclear factor-kappa B activation. Furthermore, topical RbGDE treatment reduced the AD features such as thickened skin, erythema, immune cells infiltration (eosinophils and mast cells), and AD-related cytokines (IL-12a, IL-1β, IL-4, and TSLP) in the ear tissues of DNCB/DfE-induced mice. The RbGDE also reduced histamine and immunoglobulins (Igs) levels in the serum, including DfE-specific IgE, total IgE, and IgG2a.

Conclusion: RbGD leaf extract had an anti-inflammatory effect on dermatitis by reducing inflammatory mediators, indicating that it might be used to treat skin disease.

Implication for health policy/practice/research/medical education:

This study provides scientific evidence that Rhododendron brachycarpum D. Don ex G. Don leaves extract has anti-inflammatory properties supported by the keratinocytes and an atopic dermatitis model. This extract has the potential to be a viable herbal formulation candidate for skin treatment and management.

Please cite this paper as: Kang J, Kim YJ, Chung JM, Kim N, Choi YA, Kim EN, et al. Anti-inflammatory effect of Rhododendron brachycarpum D. Don ex G. Don leaves extract on dermatitis. J Herbmed Pharmacol. 2023;12(1):73-83. doi: 10.34172/jhp.2023.06.

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Submitted: 20 Feb 2022
Revision: 07 Apr 2022
Accepted: 08 Apr 2022
ePublished: 31 Dec 2022
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