Effects of Zingiber officinale hydro-alcoholic extract on HMG-COA reductase level in the testis of streptozotocin-induced diabetic rats

Abstract

Introduction: Zingiber officinale Roscoe, commonly known as ginger, is used as a cooking spice and therapeutically for its antioxidant and androgenic activities. We investigated the effects of Z. officinale hydro-alcoholic extract on HMG-CoA (3-hydroxy-3-methylglutaryl coenzyme A) reductase level in the testis of streptozotocin (STZ)-induced diabetic rats. Methods: The current experimental study was performed on four groups of male Wistar rats one of them was kept as a healthy control, while the others were rendered diabetic via a single intraperitoneal injection of STZ (60 mg kg-1). One group was considered as diabetic control; while the others were given orally hydro-alcoholic extract (200 and 400 mg kg-1) for 56 consecutive days. Body weight, blood glucose and insulin concentrations were evaluated using standard methods. The HMG-COA reductase level was determined by western blot analysis. Results: Treatment with the extract resulted in a significant reduction of serum glucose concentration and HMG-COA reductase level in the rat’s testis compared to diabetic controls (P < 0.01). A significant increase in body weight was observed in treated diabetic rats. Also, serum insulin was significantly increased in diabetic rats treated with 400 mg/kg of the extract compared to diabetic controls (P < 0.05). Conclusion: Ginger has a potential influence on the regulation of cholesterol homeostasis by modulating of HMG-COA reductase level. The results provide scientific evidence to confirm the traditional use of Z. officinale in the treatment of diabetes mellitus.

Keywords: Streptozotocin-induced diabetes, HMG-COA reductase, Zingiber officinale, Medicinal plant