Sri Utami
1*, Qomariyah Romadhiyani Sachrowardi
1 
, Ndaru Andri Damayanti
1, Arroyan Wardhana
1, Irfan Syarif
1, Said Nafik
2, Betharie Cendera Arrahman
3, Hanna Sari Widya Kusuma
4, Wahyu Widowati
51 Faculty of Medicine, YARSI University, Central Jakarta, Indonesia
2 Directorate General of Intellectual Property, Ministry of Law and Human Rights, Republic of Indonesia, South Jakarta, Indonesia
3 Faculty of Business and Engineering, University of Applied Sciences Würzburg-Schweinfurt, Schweinfurt, Germany
4 Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia
5 Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia
Abstract
Introduction: Nitric oxide (NO) is a free radical that belongs to reactive nitrogen species (RNS). The excess amount of NO in body generates physical changing on skin as a consequence of alteration in connective tissue through formation of lipid peroxides, cell content, and enzymes. These free radicals induce damage to extracellular matrix (ECM) and are responsible in reducing skin elasticity. Antioxidants possess significant role in delaying aging process by scavenging free radicals and preventing collagenase and elastase enzymes activities. This study aimed to evaluate antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit (GpKar) as antiaging remedy. Methods: Antioxidant activity was performed by NO scavenging activity assay, while anti-aging activity was performed through inhibitory effects of collagenase and elastase activities. Results: In antioxidant activity, GpKar had lower NO scavenging activity (IC50=1530.34 μg/mL) compared to xanthone (IC50=85.40 μg/mL). In collagenase inhibitory activity, GpKar also had lower inhibition collagenase activity (IC50= 1169.31 μg/mL) compared to xanthone (IC50= 286.32 μg/mL). In elastase inhibitory activity, GpKar had lower inhibition elastase activity (IC50= 152.93 μg/mL) compared to xanthone (IC50= 21.26 μg/mL). Conclusion: In summary, GpKar and its compounds possess antioxidant, anticollagenase, and antielastase activities for antiaging, and might be beneficial in these subjects.