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J Herbmed Pharmacol. 2025;14(4): 435-446.
doi: 10.34172/jhp.2025.52961
  Abstract View: 23
  PDF Download: 14

Original Article

Phytochemical, pharmacognostic, and in silico investigation of the anti-diabetic potential of Elaeocarpus angustifolius seeds

Manasa Gopal 1 ORCID logo, Santhosh Gangaraja 1* ORCID logo, Abhishek Gollahali Shivakumar 1 ORCID logo, Pradeep Lokesh 1 ORCID logo, Niranjan Rangaswamy 1 ORCID logo, Suhas Gowda Hadenahalli Devaraju 1 ORCID logo, Bhoomika Shashidhar 1 ORCID logo, Sanjay Sanjay 1 ORCID logo

1 Department of Pharmacology, KLE College of Pharmacy, Rajajinagar, Bangalore – 560010, Karnataka, India
*Corresponding Author: Santhosh Gangaraja, Email: santhoshprince89072@gmail.com

Abstract

Introduction: Elaeocarpus angustifolius has been traditionally used for hyperglycemia, but most of its pharmacognostic and pharmacological properties remain underexplored. This study provided an evaluation of integrating pharmacognostic, physicochemical and phytochemical analyses of its seeds with network pharmacology to explore its potential in type 2 diabetes mellitus (T2DM) management.

Methods: Microscopic analysis was conducted to confirm species authentication. Physicochemical parameters, including moisture content, total ash, and acid-insoluble ash, were assessed. Phytochemical screening detected bioactive compounds, and thin-layer chromatography detected the presence of quercetin. Network pharmacology analysis was performed to predict potential metabolic and inflammatory pathways influenced by key bioactive compounds. Pathway enrichment, gene-disease association and Reactome pathway analyses were used to determine molecular targets relevant to glucose metabolism and insulin sensitivity.

Results: Microscopic analysis confirmed species authentication through the presence of prism crystals, sclereids, and oil glands. Physicochemical evaluation indicated low moisture content (6.5%), suggesting good stability, while total ash (3.2%) and acid-insoluble ash (0.8%) supported the sample’s purity. The presence of alkaloids, steroids, flavonoids, and phenols was discovered using phytochemical screening. Network pharmacology analysis demonstrated significant pathway enrichment in PI3K/Akt (P = 0.003), AMPK (P = 0.007), insulin (P = 0.012), and TNF signalling (P = 0.015) pathways. Further gene enrichment revealed MAPK1, PIK3R2, and NFKB1 as key targets significantly associated with T2DM (P < 0.05), compared to expected random gene-pathway associations.

Conclusion: This study revealed E. angustifolius as a promising candidate for diabetes management through its influence on key metabolic pathways. However, experimental and clinical trials should confirm its usage.


Implication for health policy/practice/research/medical education:

The study emphasizes the therapeutic potential of Elaeocarpus angustifolius in diabetes management. In clinical practice, the identified bioactive compounds may support the development of complementary therapies for metabolic disorders. For research, the findings provide a basis for further in vivo and clinical studies to validate its pharmacological effects. In medical education, integrating network pharmacology and phytochemical analysis into curricula can enhance understanding of natural compounds in disease management.

Please cite this paper as: Gopal M, Gangaraja S, Shivakumar AG, Lokesh P, Rangaswamy N, Devaraju SGH, et al. Phytochemical, pharmacognostic, and in silico investigation of the anti-diabetic potential of Elaeocarpus angustifolius seeds. J Herbmed Pharmacol. 2025;14(4):435-446. doi: 10.34172/jhp.2025.52961.

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Submitted: 12 Feb 2025
Revision: 10 Apr 2025
Accepted: 20 Apr 2025
ePublished: 01 Oct 2025
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