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J Herbmed Pharmacol. 2024;13(4): 651-658.
doi: 10.34172/jhp.2024.52596
  Abstract View: 27
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Original Article

The modulatory effects of Lonicera caerulea L. extract and omega-3 on sarcopenia via regulating PI3K and FOXO signaling pathways in rats

Radwa Wahid Mohamed 1* ORCID logo, Nourhan Gamal El-Rahmany 1 ORCID logo

1 Department of Biochemistry and Nutrition, Faculty of Women for Arts Science and Education, Ain Shams University, Cairo, Egypt
*Corresponding Author: Radwa Wahid Mohamed, Email: radwa.wahid@women.asu.edu.eg

Abstract

Introduction: Sarcopenia is an inflammatory disease caused by a disruption of muscle homeostasis. Lonicera caerulea L. (Haskap berry) (HB) extract and omega-3 (ω−3) possess antioxidant and anti-inflammatory activities. This study assesses the potential ameliorating effects of HB extract and ω−3 supplementation on dexamethasone (DEXA)-induced sarcopenia.

Methods: Rats were divided into five groups; the negative control group was injected with saline (i.p.); groups 2, 3, 4, and 5 were injected with DEXA (2 mg/kg/d, i.p.); groups 3 and 4 also received 400 mg/kg/d and 100 mg/kg/d of HB extract and ω−3, respectively, while group 5 received both treatments daily for 21 days. The ameliorative effects of treatments were investigated by measuring lysosomal proteolytic enzyme cathepsin activity, phosphatidylinositol 3 kinase (PI3K) activity, nuclear factor kappa beta (NF-κB) level, and heme oxygenase-1 (HO-1) activity. The gene expression levels of muscle ring-finger protein (MuRF) and forkhead box O (FOXO) transcription factor were also evaluated. Biochemical and histological examinations were done on muscle tissues.

Results: DEXA caused a significant elevation (P<0.05) in NF-κβ level and cathepsin activity in muscle tissue. Also, it significantly upregulated the muscle atrophy markers. Meanwhile, PI3K and HO-1 activities were significantly reduced. HB extract and ω−3 administration significantly (P≤0.05) reversed these effects and downregulated the mRNA expression levels of MuRF and FOXO. Also, the histopathological examination confirmed these results.

Conclusion: The current study proved the ameliorative effects of HB extract and ω−3 on sarcopenia parameters. Therefore, they might be potential therapeutic agents for myopathy/sarcopenia.


Implication for health policy/practice/research/medical education:

Lonicera caerulea (haskap berry) extract and omega-3 reversed Dexamethasone-induced myopathies. Hence, they might be recommended to ameliorate sarcopenia by modulating muscle atrophy-related genes and oxidative stress.

Please cite this paper as: Mohamed RW, EL-Rahmany NG. The modulatory effects of Lonicera caerulea L. extract and omega-3 on sarcopenia via regulating PI3K and FOXO signaling pathways in rats. J Herbmed Pharmacol. 2024;13(4):651-658. doi: 10.34172/jhp.2024.52596.

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Submitted: 07 Jun 2024
Accepted: 10 Sep 2024
ePublished: 01 Oct 2024
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