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J Herbmed Pharmacol. 2024;13(4): 620-629.
doi: 10.34172/jhp.2024.52543
  Abstract View: 34
  PDF Download: 43

Original Article

Effect of Cucurbita pepo (Pumpkin) seed extracts on the cyclicity and reproductive hormones of female Wistar rats

Chinwe Fiona Anyanwu* ORCID logo, Owunari Abraham Georgewill ORCID logo, Udeme Owunari Georgewill ORCID logo

1 Department of Pharmacology, Faculty of Basic Clinical Sciences, University of Port Harcourt, P.M.B. 5323, Nigeria
*Corresponding Author: Chinwe Fiona Anyanwu, Email: Chinwe.anyanwu@uniport.edu.ng

Abstract

Introduction: Despite the traditional use of Cucurbita pepo seed in pregnancy, its effects on female reproduction remain scarce. This study evaluated the impacts of n-hexane, dichloromethane (DCM), and aqueous ethanol extracts of C. pepo seed on the cyclicity and reproductive hormones of female Wistar rats.

Methods: Ten groups of four rats received seed extracts or tween 80 orally for 21 days: A (control)= 0.5 mL tween 80 (vehicle); B, C, & D= 142.86, 285.71, and 428.57 mg/kg nHE; E, F, & G= 142.86, 285.71, 428.57 mg/kg of DCM; and H, I, & J= 142.86, 285.71, 428.57 mg/kg of aqueous ethanol extracts, respectively. Vaginal cytology monitored the estrous cycle daily, and blood samples were obtained for follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen, and progesterone at various oestrus cycle phases.

Results: Compared to the control, the estrous cycle phases did not change significantly (P>0.05). FSH levels significantly increased (P<0.05) with DCM and aqueous ethanol extracts of C. pepo seed during proestrus and estrus phases compared to the control. A significant (P<0.05) increase in LH was observed with n-hexane, DCM, and aqueous ethanol extracts during all estrous cycle phases compared to the control. All extracts significantly increased estrogen levels (P<0.05) during all phases. DCM and aqueous ethanol extracts reduced substantially the estrus-phase progesterone.

Conclusion: Cucurbita pepo may stimulate the hypothalamic-pituitary-gonadal axis in female reproduction. Further studies should be conducted using various phytoestrogen compounds to gain useful knowledge about the effectiveness, safety, and long-term effects of C. pepo seed extracts in regulating hormonal balance.


Implication for health policy/practice/research/medical education:

This article provides scientific evidence of the stimulatory effect of Cucurbita pepo seed on the hypothalamic-pituitary-gonadal axis in female reproduction. Thus, this could inform healthcare practitioners to assess natural dietary supplements as part of a holistic female reproductive health strategy.

Please cite this paper as: Anyanwu CF, Georgewill OA, Georgewill UO. Effect of Cucurbita pepo (Pumpkin) seed extracts on the cyclicity and reproductive hormones of female Wistar rats. J Herbmed Pharmacol. 2024;13(4):620-629. doi: 10.34172/jhp.2024.52543.

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Submitted: 11 Apr 2024
Accepted: 30 Jun 2024
ePublished: 01 Oct 2024
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