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J Herbmed Pharmacol. 2024;13(1): 90-100.
doi: 10.34172/jhp.2024.48116
  Abstract View: 160
  PDF Download: 154

Original Article

Magnolia kobus DC leaf ethanol extract alleviated lipopolysaccharide-induced acute lung inflammation by suppressing NF-κB and Nrf2 signaling

Yeyoung Kim 1 ORCID logo, Soyoung Lee 2 ORCID logo, Young-Ae Choi 1 ORCID logo, Jae-Min Chung 3 ORCID logo, Eun-Nam Kim 4 ORCID logo, Byungheon Lee 5 ORCID logo, Sang-Yong Kim 6* ORCID logo, Gil-Saeng Jeong 4* ORCID logo, Sang-Hyun Kim 1* ORCID logo

1 Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 41944, South Korea
2 Immunoregulatory Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, South Korea
3 Department of Gardens Education, Korea National Arboretum, Pocheon 11186, South Korea
4 College of Pharmacy, Chungnam National University, Daejeon 34134, South Korea
5 Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu 41944, South Korea
6 DMZ Botanic Garden, Korea National Arboretum, Yanggu 24564, South Korea
*Corresponding Authors: *Corresponding author: Sang-Yong Kim, Email: , Email: rosaksy@korea.kr; *Corresponding author: Gil-Saeng Jeong, Email: , Email: gsjeong@cnu.ac.kr; *Corresponding author: Sang-Hyun Kim, Email: , Email: shkim72@knu.ac.kr

Abstract

Introduction: Magnolia kobus DC has been used as herbal medicine to treat coughs and is known to exert biological effects such as anti-inflammatory, antioxidant, and antibacterial properties. We aimed to define the pharmacological effects of M. kobus leaf ethanol extract (MLEE) on acute lung inflammation and explore the underlying mechanisms of action.

Methods: For in vitro investigations, RAW 264.7 cells were pretreated with MLEE (1, 10, and 100 μg/mL) and stimulated with lipopolysaccharide (LPS). For in vivo investigations, BALB/c mice were intratracheally administered with LPS for 24 hours after injection of MLEE (0.3, 3, and 30 mg/kg). Hematoxylin and eosin staining was used for histopathology analysis of lung tissue. The phytochemical constituents of MLEE were analyzed using high-performance liquid chromatography.

Results: In RAW 264.7 cells, MLEE reduced the activation of the inflammatory mediators (inducible nitric oxide synthase and cyclooxygenase-2) and the nuclear translocation of nuclear factor (NF)-κB and nuclear factor erythroid-2-related factor 2 (Nrf2). The intraperitoneal injection of MLEE (30 mg/kg) attenuated interstitial edema and immune cell infiltration in LPS-induced acute lung inflammation. MLEE also inhibited the activation of cyclooxygenase-2, NF-κB, and Nrf2 in the lung tissue.

Conclusion: Taken together, MLEE exerted an anti-inflammatory effect by inhibiting inflammatory and oxidative mediators on acute lung inflammation suggesting that it might be used as a natural drug for treating acute lung inflammatory diseases.


Implication for health policy/practice/research/medical education:

The extract of Magnolia kobus leaf has anti-inflammatory and antioxidative effects evidenced by the activated macrophages and acute lung inflammation mouse model. This suggests potential as a natural product candidate for the remedy of acute lung inflammation.

Please cite this paper as: Kim Y, Lee S, Choi YA, Chung JM, Kim EN, Lee B, et al. Magnolia kobus DC leaf ethanol extract alleviated lipopolysaccharide-induced acute lung inflammation by suppressing NF-κB and Nrf2 signaling. J Herbmed Pharmacol. 2024;13(1):90-100. doi: 10.34172/jhp.2024.48116.

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Submitted: 26 May 2023
Revision: 25 Jul 2023
Accepted: 25 Jul 2023
ePublished: 01 Jan 2024
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