Logo-jhp
J Herbmed Pharmacol. 2024;13(1): 74-79.
doi: 10.34172/jhp.2024.48105

Scopus ID: 85185762941
  Abstract View: 397
  PDF Download: 277

Original Article

Comparison of the protective effects of insulin and saponin on renal ischemia-reperfusion injury

Fardin Rezaei 1 ORCID logo, Mohammad Ghasem Golmohammadi 2 ORCID logo, Hakimeh Saadati 1 ORCID logo, Shokofeh Banaei 1* ORCID logo

1 Department of Physiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
2 Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
*Corresponding Author: *Corresponding author: Shokofeh Banaei, Email: , Email: S.banaei75@gmail.com

Abstract

Introduction: Oxidative stress induced by renal ischemia-reperfusion (IR) is an effective element in the pathophysiologic renal alterations generated during IR injury. This investigation intended to study the effects of saponin (SP) and insulin (INS) in kidney IR injury.

Methods: Wistar male rats were assigned into four groups: the control, IR, SP + IR, and INS + IR. The rats were nephrectomized unilaterally and went under 45 minutes ischemia followed by 24-hour reperfusion. SP (2.5 mg/kg) and INS (30 U/kg) were intraperitoneally injected prior to ischemia. Following the treatment, a blood sample was taken for the measurement of biochemical parameters, and the left kidney was taken out for the determination of oxidative stress markers and histological changes.

Results: Blood urea nitrogen (BUN), creatinine (Cr), malondialdehyde (MDA) levels, and histopathological scores significantly increased by kidney IR while antioxidant enzymes were significantly decreased. However, the administration of SP and INS significantly decreased BUN, Cr, MDA, and histological scores and improved the antioxidant defense system.

Conclusion: SP and INS exert nephroprotective effects against oxidative damage caused by IR. It seems that SP has a renoprotection similar to INS in renal IR.


Implication for health policy/practice/research/medical education:

Insulin and saponin have antioxidant properties and might be used to protect ischemia reperfusion-induced renal damage.

Please cite this paper as: Rezaei F, Golmohammadi MG, Saadati H, Banaei S. Comparison of the protective effects of insulin and saponin on renal ischemia-reperfusion injury. J Herbmed Pharmacol. 2024;13(1):74-79. doi: 10.34172/jhp.2024.48105.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 381

Your browser does not support the canvas element.


PDF Download: 277

Your browser does not support the canvas element.

Submitted: 12 Jun 2023
Revision: 20 Aug 2023
Accepted: 20 Aug 2023
ePublished: 01 Jan 2024
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)