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J Herbmed Pharmacol. 2024;13(2): 216-225.
doi: 10.34172/jhp.2024.46051

Scopus ID: 85192248386
  Abstract View: 736
  PDF Download: 467

Original Article

Effects of hydroethanolic garlic extract on oxidative stress, lipolysis, and glycogenesis in high-fat diet-fed Drosophila melanogaster

Ayodeji E. Adepoju 1,2 ORCID logo, Monde Ntwasa 3 ORCID logo, Sogolo L. Lebelo 3 ORCID logo, Temitope A. Oyedepo 2 ORCID logo, Ademola O. Ayeleso 3,4* ORCID logo

1 Department of Nutrition and Exercise Physiology, College of Agriculture, Food and Natural Resources, School of Medicine, University of Missouri-Columbia, USA
2 Department of Biochemistry, Adeleke University, Ede, Nigeria
3 Department of Life and Consumer Sciences, University of South Africa, Florida Park, Roodepoort, South Africa
4 Biochemistry Programme. College of Agriculture, Engineering and Science, Bowen University Iwo, Nigeria
*Corresponding Author: Email: ademola.ayeleso@bowen.edu.ng

Abstract

Introduction: Metabolic syndrome is multifaceted health condition associated with metabolism, which leads to the accumulation of oxidation products and impairment of the antioxidant system. Medicinal, edible plant alternatives are being sought to prevent the predisposing factors of metabolic syndrome, one of which is obesity. We studied the effects of hydroethanolic garlic extract (HGE) on oxidative stress, lipolysis, and glycogenesis in high-fat diet-fed Drosophila melanogaster.

Methods: The HGE was processed and antioxidant assays (in vitro and in vivo) coupled with metabolic biomarkers were assayed following standard procedures.

Results: HGE inhibited ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), hydroxyl, and hydrogen peroxide radicals in high-fat-fed D. melanogaster. The HGE also reduced the activity of superoxide dismutase and increased catalase activity significantly. The HGE promoted lipolysis and glycogenesis by reducing cholesterol, triglycerides, and total sugar concentrations while glycogen content was increased.

Conclusion: Our results revealed that HGE improved the antioxidant defense system and promoted lipolysis and glycogenesis in metabolic deranged D. melanogaster. This suggests the therapeutic usefulness of garlic in the management of metabolic diseases.


Implication for health policy/practice/research/medical education:

Hydroethanolic garlic extract improved the antioxidant defense system in metabolic deranged Drosophila melanogaster by scavenging of free radicals and modulation of antioxidant enzymes. The garlic extract also promoted lipolysis and glycogenesis, thereby ameliorating obesity. This indicates that garlic or its compo-nents might be used for obesity.

Please cite this paper as: Adepoju AE, Ntwasa M, Lebelo SL, Oyedepo TA, Ayeleso AO. Effects of hydroethanolic garlic extract on oxidative stress, lipolysis, and glycogenesis in high-fat diet-fed Drosophila melanogaster. J Herbmed Pharmacol. 2024;13(2):216- 225. doi: 10.34172/jhp.2024.46051.

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Submitted: 05 May 2023
Accepted: 08 Mar 2024
ePublished: 01 Apr 2024
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