J Herbmed Pharmacol. 2024;13(3): 399-406.
doi: 10.34172/jhp.2024.44959
  Abstract View: 73
  PDF Download: 83

Original Article

Cytotoxicity and antimycobacterial activity of fractions and chemical constituents of Lecaniodiscus cupanioides Planch. Ex Benth

Olusesan Ojo 1* ORCID logo, Michael H.K. Kamdem 1 ORCID logo, Edwin M. Mmutlane 2 ORCID logo, Derek T. Ndinteh 1* ORCID logo

1 Centre for Natural Product Research (CNPR), Chemical Sciences Department, University of Johannesburg, Doornfontein, Johannesburg 2028, South Africa
2 Research Centre for Synthesis and Catalysis, Department of Chemical Sciences, University of Johannesburg, Kingsway Campus, Auckland Park, P.O. Box 524, Johannesburg, South Africa
*Corresponding Authors: Olusesan Ojo, Email: dndinteh@uj.ac.za, Email: Ojoolusesan33@gmail.com; Derek T. Ndinteh, Email: dndinteh@uj.ac.za


Introduction: Mycobacterial infections remain a global problem that demands an urgent solution. Lecaniodiscus cupanioides Planch. ex Benth. is traditionally used as a medicinal plant to treat coughs, skin infections, cancers, sexual dysfunction, and malaria. This study focused on the antimycobacterial activity, cytotoxicity, and isolation of bioactive constituents of L. cupanioides chloroform leaves extract.

Methods: Antimycobacterial activity was assessed using the micro-dilution assay. Cytotoxic activity of the plant was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay. Column chromatography was used to purify the extract.

Results: Phytochemical investigation of L. cupanioides resulted in the isolation of three compounds, including eicosene (1), nonadecanol (2), and stigmasterol (3). The isolates 1-3 as well as the column fractions displayed antimycobacterial activity, with minimum inhibitory concentration (MIC) ranging from 0.50 to 1.0 mg/mL. The studied extract, column fractions, and isolated compounds exhibited no cytotoxicity against the human cervical cancer (HeLa) cell line.

Conclusion: The current results indicate that L. cupanioides has the potential to be employed in the formulation of herbal products or the development of new drugs against mycobacterial infections. However, more studies should be carried out using normal human cell lines to further unravel plant safety.

Implication for health policy/practice/research/medical education:

The phytochemical constituents of chloroform leaf extract of Lecaniodiscus cupanioides were identified, isolated, and discovered to have antimycobacterial properties, especially for managing infections, such as skin or soft-tissue, pneumonia, bacteremia, and bone diseases clinically caused by Mycobacterium smegmatis.

Please cite this paper as: Ojo O, Kamdem MHK, Mmutlane ED, Ndinteh DT. Cytotoxicity and antimycobacterial activity of fractions and chemical constituents of Lecaniodiscus cupanioides Planch. Ex Benth. J Herbmed Pharmacol. 2024;13(3):399-406. doi: 10.34172/jhp.2024.44959.

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Submitted: 29 Mar 2023
Accepted: 09 Aug 2023
ePublished: 27 Jun 2024
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