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J Herbmed Pharmacol. 2024;13(1): 43-51.
doi: 10.34172/jhp.2024.44523
  Abstract View: 217
  PDF Download: 152

Original Article

Antioxidant capacities, antidiabetic potentials, and mineral compositions of pap aqua and aqueous extracts from Ocimum gratissimum L.

Oludare Shadrach Agunbiade 1 ORCID logo, Olakunle Bamikole Afolabi 1* ORCID logo, Kikelomo Folake Jaiyesimi 1 ORCID logo, Olusola Bolaji Adewale 1 ORCID logo, Patrick Osemeyekeh Okoh 1 ORCID logo, Ezekiel Adewole 2 ORCID logo, Samson Olatunde Mabayoje 3 ORCID logo, Esther Kemi Agboola 1 ORCID logo

1 Phytomedicine Unit, Biochemistry Programme, Department of Chemical Sciences, College of Sciences, Afe Babalola University, P.M.B. 5454, Ado‐Ekiti, Ekiti State, Nigeria
2 Industrial Chemistry Programme, Department of Chemical Sciences, College of Sciences, Afe Babalola University, P.M.B. 5454, Ado‐Ekiti, Ekiti State, Nigeria
3 Department of Biological Sciences, College of Sciences Afe Babalola University, Km 8.5, Afe Babalola Way, P.M.B. 5454, Ado‐Ekiti, Ekiti State, Nigeria
*Corresponding Author: *Corresponding author: Olakunle Bamikole Afolabi, Email:, Email: afolabiob@abuad.edu.ng

Abstract

Introduction: This study appraised the antioxidant potentials, mineral compositions, and antidiabetic activities of pap water (aqua) extract (PWE) and aqueous extract (AE) from Ocimum gratissimum.

Methods: The total phenolic contents (TPC), total flavonoid contents (TFC), ferric reducing antioxidant powers (FRAP), Fe2+-chelating abilities, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging abilities, and enzymes inhibition potentials of the PWE and AE from O. gratissimum were evaluated. Additionally, mineral contents were determined using absorptive absorbance spectroscopy (AAS).

Results: The PWE of O. gratissimum exhibited higher TPC of 42.61± 0.04 mg gallic acid equivalent per gram of dried sample (GAE/g) and TFC of 85.7 ± 0.02 µg quercetin equivalent per gram of dried sample (QE/g). In contrast, AE had lower TPC (21.52 ± 0.01 mg GAE/g) and TFC (55.0 ± 0.01 µg QE/g). PWE also displayed a lower FRAP of 2.86 ± 0.01 mg AAE/g, while AE had a higher FRAP of 2.94 ± 0.03 mg AAE/g. PWE of O. gratissimum had IC50 for DPPH: 100.00 µg/mL, Fe2+-chelating ability: 4.41 µg/mL, while AE had IC50 for DPPH: 140.00 µg/mL and Fe2+-chelating ability: 4.90 µg/mL. Similarly, the PWE of O. gratissimum showed a higher α-amylase inhibition (IC50: 0.47 mg/mL) than AE (IC50: 0.78 mg/mL); however, AE (IC50 =3.09 µg/mL) demonstrated a higher α-glucosidase inhibition than PWE (IC50: 9.09 µg/mL). AAS analyses indicated the presence of Ca, Fe, Mg, Cu, Zn, and Mn in different proportions in both extracts.

Conclusion: Therefore, PWE could be a better alternative in the management of diabetes melitus if properly annexed.


Implication for health policy/practice/research/medical education:

Pap water and aqueous extracts of Ocimum gratissimum demonstrate significant antioxidant and antidiabetic potentials, as well as mineral compositions, which might be useful as clinical therapy in hyperglycemia.

Please cite this paper as: Agunbiade OS, Afolabi OB, Jaiyesimi KF, Adewale OB, Okoh PO, Adewole E, et al. Antioxidant capacities, antidiabetic potentials, and mineral compositions of pap aqua and aqueous extracts from Ocimum gratissimum L. J Herbmed Pharmacol. 2024;13(1):43-51. doi: 10.34172/jhp.2024.44523.

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Submitted: 14 Jun 2022
Revision: 20 Nov 2022
Accepted: 21 Nov 2022
ePublished: 01 Jan 2024
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