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J Herbmed Pharmacol. 2022;11(2): 296-304.
doi: 10.34172/jhp.2022.35

Scopus ID: 85129481940
  Abstract View: 1434
  PDF Download: 786

Original Article

Hydroethanolic Cyperus rotundus L. extract exhibits anti-obesity property and increases lifespan expectancy in Drosophila melanogaster fed a high-fat diet

Nattapong Wongchum 1, 2 ORCID logo, Ananya Dechakhamphu 3, 4* ORCID logo, Panatda Panya 3, 4 ORCID logo, Somchai Pinlaor 5 ORCID logo, Sitthisak Pinmongkhonkul 6 ORCID logo, Alongklod Tanomtong 1 ORCID logo

1 Biology Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
2 Biology Program, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubonratchathani 34000, Thailand
3 Thai Traditional Medicine Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani 34000, Thailand
4 Aesthetic Sciences and Health Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani 34000, Thailand
5 Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
6 Department of Biology, School of Science, University of Phayao, Phayao 56000, Thailand
*Corresponding Author: Email: ananya.d@ubru.ac.th

Abstract

Introduction: Cyperus rotundus L. is suspected of having anti-obesity properties. The purpose of this study was to determine the anti-obesity property of hydroethanolic C. rotundus extract (HECE) using Drosophila as a model organism. Methods: In vitro inhibition of lipase activity by C. rotundus extract was investigated. The effects of C. rotundus extract on obesity-related characteristics, including body weight, triglyceride content, and lifespan extension were evaluated in Drosophila fed a high-fat diet (HFD). The effect of the extract on the reduction of oxidative stress associated with obesity was assessed in vivo using antioxidant assays in Drosophila. Results: HECE inhibited lipase activity in vitro with an IC50 of 128.24 ± 3.65 μg/mL. In vivo lipase inhibition experiments demonstrated that feeding Drosophila 10 mg/mL HECE or 2 μM orlistat lowered lipase activity by 21.51 (P < 0.05) and 42.86% (P < 0.01) and triglyceride levels by 20.67 (P < 0.05) and 28.39% (P < 0.01), respectively, compared to those of the untreated group. After 10 mg/mL HECE or 2 μM orlistat supplementation, an increase in the mean survival rate (10.54 (P < 0.05) and 13.90% (P < 0.01), respectively) and climbing ability (25.03 (P < 0.01) and 28.44% (P < 0.01), respectively) was observed compared to those of flies fed a HFD. The paraquat and H2O2 challenge tests revealed that flies fed HECE in a mixed HFD showed increased survival on flies fed a HFD. Conclusion: This study demonstrates the beneficial effects of dietary HECE supplementation on suppressing pancreatic lipase activity and lowering triglyceride levels and oxidative stress, leading to increased lifespan in Drosophila fed a HFD.

This study demonstrates the beneficial effects of dietary hydroethanolic Cyperus rotundus extract supplementation on suppressing pancreatic lipase activity and lowering triglyceride levels and oxidative stress. C. rotundus extract may be used in the future to develop an anti-obesity drug.
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Submitted: 31 Jan 2022
Revision: 09 Feb 2022
Accepted: 15 Feb 2022
ePublished: 01 Apr 2022
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