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J Herbmed Pharmacol. 2015;4(1): 35-39.
  Abstract View: 3974
  PDF Download: 2431

Original Article

Total phenolic compounds and in vitro antioxidant potential of crude methanol extract and the correspond fractions of Quercus brantii L. acorn.

Ali Karimi 1, Mohammad-Taghi Moradi 2*

1 Faculty of Paramedicine, Shahrekord University of Medical Science, Shahrekord, Iran
2 PhD student, Medical Plants Research Center, Shahrekord University of Medical Science, Shahrekord, Iran
*Corresponding Author: *Corresponding author: Mohammad-Taghi Moradi, Medical Plants Research Center, Shahrekord University of Medical Science, Rahmatieh, Shahrekord, Iran. , Email: mtmoradi65@gmail.com

Abstract

Introduction: Due to their roles in the maintenance of human health, phytochemicals and antioxidants in plants are raising interest. Phenolic and flavonoids are components with potential to protect against human diseases such as cardiovascular disease and cancers. Q. brantii is one of the traditional folk medicinal plants widely used in Iran. In this study, crude methanol extract and four correspond fractions of this plant were used to evaluate the total phenolic, total flavonoids, total flavonol, and antioxidant activities.

Methods: The identification of phenolic, flavonoid, and flavonol components and assay of antioxidant activity were carried out using standard in vitro procedures.

Results: The n-butanol fraction (376.2±7.1) had the highest amount of total phenolic compounds and the n-butanol fraction exhibited the lowest IC50 value (6.5±0.6 μg ml). There was significant relationship between the total phenolic contents and the free radical scavenging property in the four fractions in this study (R= -0.768, P< 0.01).

Conclusion: The results indicate that at least, n-butanol fraction of this plant with high phytoconstituents and less toxicity could be a promising source of medicinally important natural compound.


Implication for health policy/practice/research/medical education:

This study indicates that, at least some fractions of methanolic extract of Q. brantii acorn can be used as an accessible source of natural antioxidants. However, further works might be needed to the other extracts of this plant to reveal and evaluate its antioxidant potential.

Please cite this paper as: Karimi A, Moradi MT. Total phenolic compounds and in vitro antioxidant potential of crude methanol extract and the correspond fractions of Quercus brantii L. acorn. J Herbmed Pharmacol 2015; 4(1): 35-39.

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