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J Herbmed Pharmacol. 2014;3(1): 31-34.
  Abstract View: 3099
  PDF Download: 2361

Original Article

Screening of antibacterial effect of the Scrophularia Striata against E. coli in vitro

Reza Sharafati-chaleshtori 1, Mahmoud Rafieian-kopaei 2*

1 Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran
2 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding Author: *Corresponding author: Mahmoud Rafieian-kopaei, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. , Email: rafieian@yahoo.com

Abstract

Introduction: This study was aimed to evaluate the antimicrobial activity of the ethanol and aqueous extracts of Scrophularia striata plant on E. coli O157:H7 in vitro.

Methods: In this experimental study the ethanol and aqueous extract of the plant was prepared and their antibacterial effects were determined using sink diffusion and broth macrodilution methods against the bacterium E. coli O157:H7.

Results: The ethanol extract of Scrophularia striata plant had inhibitory effect on the E. coli O157:H7 in two methods of sink diffusion and macrodilution, but the aqueous extract of this plant had not antibacterial effect. The MIC and MBC amounts were obtained 90mg/ml and 100 mg/ml, respectively.

Conclusion: Based on the present results that the ethanol extract of the Scrophularia Striata plant showed inhibitory effect on bacterium, more researches are recommended to evaluate its in vivo effects and to identify active compounds.


Implication for health policy/practice/research/medical education:

Scrophularia striata plant showed antibacterial properties in in vitro conditions which may provide an insight for future research in order to identify its effective compounds in in vivo condition.

Please cite this paper as: Sharafati-chaleshtori R, Mahmoud Rafieian-kopaei M. Screening of antibacterial effect of the Scrophularia Striata against E. coli in vitro. J Herbmed Pharmacol. 2014; 3(1): 31-34.

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ePublished: 01 Jun 2014
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