Mehdi Ghasemi
1, Aghafakhr Mirlohi
2*, Mahdi Ayyari
1, Abdolali Shojaeiyan
11 Department of Horticulture Science, Tarbiat Modares University, Tehran, Iran
2 Department of Agronomy and Plant Breeding, Isfahan University of Technology, Isfahan, Iran
Abstract
Abstract
Introduction: The present study is the first assessment of the fatty
acids of leaf and essential oil
compositions of new three habitats of aerial parts of K. odoratissima.
Methods: The aerial parts of K.
odoratissima from the three habitats were dried. The essential oils were obtained by
hydrodistillation for 3 h in a Clevenger-type apparatus, then the analysis of the components was carried out using gas
chromatography–mass spectrometry. To study the oil yield and fatty acids, the
dried leaves subjected to extraction in hexane by using
Soxhlet Apparatus. To analyze fatty acids from the oil fractions by gas
chromatography technique, the oil was subjected to transesterification to
obtain the fatty acid methyl esters, which, were dissolved in hexane and
subjected to GC analysis.
Results: According to the results, a total of 43
components, the major constitutes of essential oil compositions were (Z)-Ligustilide
(76.45%), Unknown-A (4.47%), (E)-Ligustilide
(2.57%), (Z)-Butylidene
phthalide (2.37%), 5-pentyl cyclohexa-1,3-diene (1.57%) and Kessane (0.77%) in K. odoratissima. The sixteen fatty acids were separated from the oil (5% yield per 100 g dry matter). Linoleic
acid (25.46%), α-Linolenic acid (16.66%), Palmitic acid (11.92%), Oleic acid
(9.33%), Stearic acid (4.72%), Petroselinic acid (2.53%), Arachidonic acid (2.51%) and Erucic acid (1.76%) were
major fatty acids.
Conclusion: Generally, K. odoratissima is a rich source of essential fatty
acids and phthalide derivatives, specially (Z)-ligustilide. This study
was presented valuable information about the phytochemical properties, which
can be useful for the future researches on the pharmacological effects of K.
odoratissima.