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J Herbmed Pharmacol. 2022;11: 348-359.
doi: 10.34172/jhp.2022.40
  Abstract View: 148
  PDF Download: 90

Original Article

Antioxidant and anti-inflammatory activities of Ganoderma resinaceum (Boud) fruiting bodies extracts

Marius Trésor Kemegne Sipping 1,2 ORCID logo, Francine Kengne Mediesse 1,3 ORCID logo, Annette Yannuvie Natia Sombes 1 ORCID logo, Adamou Mfopa 1,3 ORCID logo, Thaddée Boudjeko 1,4* ORCID logo

1 Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, University of Yaoundé 1, P.O. Box 17673, Etetak, Yaoundé, Cameroon
2 Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, Yaoundé 812, Cameroon
3 Institute of Medical Research and Medicinal Plants Studies, P.O. Box. 13033, Yaoundé, Cameroon
4 Department of Biochemistry, Faculty of Sciences, University of Yaoundé 1, P.O. Box 812, Yaoundé, Cameroon
*Corresponding Author: *Corresponding author: Thaddée Boudjeko, E-mail: , Email: boudjeko@yahoo.com

Abstract

Introduction: Ganoderma resinaceum is used to treat oxidative and inflammatory-related diseases such as cardiovascular and liver diseases. Thus, this study aimed to evaluate the antioxidant and anti-inflammatory activities of different extracts from G. resinaceum fruiting bodies.

Methods: Aqueous crude (GRT), mycelial (MYC), exopolysaccharide (EPS I, EPS II) and water-soluble polysaccharide-rich (GRP I and GRP II) extracts of G. resinaceum were assessed for their free radical scavenging and metal chelating ions assays. The in vitro anti-inflammatory activity was evaluated by stabilization of erythrocytes’ membranes and protein denaturation assays. For the in vivo study, paw oedema was induced by administration of κ-carrageenan (0.1 mL; 1%) to male Wistar rats aged 4 to 6 weeks. Animals were pre-treated with G. resinaceum extracts (125 mg/kg) and diclofenac sodium (20 mg/kg). Inflammatory cytokine and chemokine levels were determined, and histological analysis of paw tissue was performed.

Results: G. resinaceum polysaccharide-rich extracts (GRP I and GRP II) showed the best bioactivities. They scavenged DPPH (1,1-diphenyl-2-picrylhydrazyl, ABTS (2,2-azino-bis-3- ethylbenzylthiazoline-6-sulfonic acid, and NO (nitric oxide) radicals, and chelated ferrous ions, stabilized murine erythrocyte membranes, and inhibited protein denaturation. At 125 mg/kg, GRP I and GRP II restored the microarchitecture with a weak infiltration of immune cells in the subcutaneous tissues. Moreover, they decreased the overproduction of proinflammatory cytokines growth colony-stimulating factor (G-CSF), interferon gamma (IFNγ), tumour necrosis factor alpha (TNFα), chemokines (eotaxin, fractalkine) and increased the levels of anti-inflammatory cytokines (IL-10, IL-12p70).

Conclusion: G. resinaceum polysaccharide extracts could be potent antioxidant and antiinflammatory agents.

Keywords: Ganoderma resinaceum, Antioxidant, Inflammation, Cytokines, Chemokines

Implication for health policy/practice/research/medical education: Research on safe and novel potential antioxidant and anti-inflammatory agents from medicinal plants such as mushrooms is necessary to palliate the side effects of anti-inflammatory drugs in long-term use. This article valorises G. resinaceum polysaccharide-rich extracts as an alternative phytodrug for this purpose. Please cite this paper as: Sipping KMT, Mediesse KF, Sombes AYN, Mfopa A, Boudjeko T. Antioxidant and anti-inflammatory activities of Ganoderma resinaceum (Boud) fruiting bodies extracts. J Herbmed Pharmacol. 2022;11(3):348-359. doi: 10.34172/ jhp.2022.40.
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Submitted: 09 Nov 2021
Revision: 17 Jan 2022
Accepted: 21 Jan 2022
ePublished: 25 Jun 2022
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