Logo-jhp
  Abstract View: 38

Original Article

Protective effects of black bamboo leaves on the glucose-induced toxicity in Caenorhabditis elegans

Eun Hye Park 1 ORCID logo, Hong Jun Kim 2 ORCID logo, Dong Seok Cha 1* ORCID logo

1 College of Pharmacy, Woosuk University, Jeonbuk 55338, Republic of Korea
2 Department of Korean Medicine, Woosuk University, Jeonbuk 55338, Republic of Korea

Abstract

Introduction: Previous studies have suggested that high glucose (HG) condition shortens the lifespan of worms by increasing glycolytic flux followed by accelerating reactive oxygen species (ROS) production, termed glucose toxicity. This study was designed to investigate the protective effects of the leaves of black bamboo (Phyllostachys nigra var. henosis) on the HG-induced toxicity using Caenorhabditis elegans model system. Methods: To determine the effect of black bamboo leaf extract (BLE) against HG-induced toxicity, lifespan assay was carried out with wild-type and daf-16 null mutant worms under 2% glucose condition. The involvement of DAF-16 was further confirmed by observing fluorescence signal of transgenic mutant carrying DAF-16::GFP transgene. ROS levels and glucose concentration of worms were analyzed using fluorescent probe H2DCF-DA and glucose meter, respectively. Nile-red staining was carried out to evaluate the lipid storage of worms. Intercellular lipid accumulation was measured using Oil-Red O staining method. Results: BLE strongly extended the lifespan of worms under not only normal culture condition but also HG condition. Our additional studies suggested that DAF-16 activation was responsible for BLE-mediated longevity and protective action against glucose toxicity. In addition, HG-fed worms showed increased ROS generation, and it was completely normalized by BLE treatment. Moreover, BLE reduced body glucose concentration and lipid accumulation in HG-fed worms. We further confirmed the inhibitory effect of BLE on lipid storage under HG condition using 3T3-L1 adipocytes. Conclusion: These therapeutic values of BLE on glucose toxicity raise the possibility that BLE might have beneficial effects on the pathogenesis of diabetes mellitus.
Keywords: Phyllostachys nigra, Black bamboo, Glucose toxicity, Diabetes mellitus, Caenorhabditis elegans
Black bamboo leaves showed potent protective potential against glucose toxicity and thereby could be suggested as a candidate in the development of a new therapeutic agent for diabetic mellitus and its complications.
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 38

Your browser does not support the canvas element.


PDF Download: 0

Your browser does not support the canvas element.

Submitted: 15 Jun 2020
Revision: 20 Aug 2020
Accepted: 20 Aug 2020
ePublished: 07 Jan 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)