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Original Article

T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice

Wira Eka Putra 1, 2* ORCID logo, A. Rofiqi Maulana 3 ORCID logo, Arfan Tri Kusuma Ramadhan 4 ORCID logo, Muhaimin Rifa’i 4 ORCID logo

1 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia
2 Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Indonesia
3 Department of Statistics, Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia
4 Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia

Abstract

Introduction: Sambucus is a genus of the Adoxaceae family and has been widely used as a food and medicinal source. In this study, we aimed to evaluate the effects of S. javanica extracts toward T cells regulation in the 7,12-dimethylbenzene (a) anthracene (DMBA)-exposed mice.Methods: Thirty mice were used in this experiment which divided into six treatment groups and five times repetition. Three-month-old BALB/c mice were administrated with 2.8 mg.kg-1 BW of DMBA as carcinogen agent for ten times within four weeks, then continuously treated with 400 and 800 mg.kg-1 BW of S. javanica berries leaves extracts daily for two weeks. Treatment groups in this study were vehicle as negative control, DMBA 2.8 mg.kg-1 BW as positive control; BD1 group, DMBA 2.8 mg.kg-1 + berries extracts 400 mg.kg-1 BW; BD2 group, DMBA 2.8 mg.kg-1 + berries extracts 800 mg.kg-1 BW; LD1 group, DMBA 2.8 mg.kg-1 + leaves extract 400 mg.kg1 BW; and LD2 group, DMBA 2.8 mg.kg-1 + leaves extract 800 mg.kg-1 BW. The relative number of CD4+CD25+CD62L+ cells and CD4+CD62L+ cells were measured and analyzed by using flow cytometer.Results: DMBA induction decreased the relative number of CD4+CD25+CD62L+ cells while the leaves extract of S. javanica significantly increased their expression. Furthermore, DMBA promoted the relative number of CD4+CD62L+ cells expression while leaves and berries extracts of S. javanica sharply suppressed its expression.Conclusion: The results suggest that, S. javanica extracts may exert their medicinal properties by modulating T cells regulation in DMBA-induced mice.
Keywords: DMBA, Naive T cells, Regulatory T cells, S. javanica

Implication for health policy/practice/research/medical education: Berries and leaves extracts of Sambucus javanica modulated the T cells regulation by increasing the relative number of CD4+CD25+CD62L+ regulatory T cells and decreasing the relative CD4+CD62L+ cells after exposed by DMBA. These results suggest that Sambucus javanica has therapeutical potential against the unfavorable effects of DMBA by modulating the T cells regulation.

Please cite this paper as: Putra WE, Maulana AR, Ramadhan ATK, Rifa’i M. T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice. J Herbmed Pharmacol. 2020;9(4):408-411. doi: 10.34172/jhp.2020.51.

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Submitted: 01 Sep 2018
Accepted: 17 May 2019
ePublished: 01 Jul 2020
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