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J Herbmed Pharmacol. 2022;11: 409-418.
doi: 10.34172/jhp.2022.47
  Abstract View: 117
  PDF Download: 49

Original Article

Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus

Stephen Kayode S. Ojo 1* ORCID logo, Gabriel Temitope Sunmonu 1 ORCID logo, Akinwunmi O. Adeoye 2 ORCID logo, Christiana Fisayo Akinwunmi 1 ORCID logo, Moses Ifeoluwa Obakunle 1 ORCID logo, Amos Olakunle Ojerinde 3 ORCID logo, Oluwakemi J. Awakan 4 ORCID logo

1 Drug Discovery and Infectious Diseases Research Group, Department of Microbiology, Federal University Oye-Ekiti, Ekiti State, Nigeria
2 Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria
3 Faculty of Basic Medical Sciences, Federal University Oye-Ekiti, Ekiti State, Nigeria
4 Department of Biochemistry, Landmark University, Omu-Aran, Kwara-State, Nigeria
*Corresponding Author: *Corresponding author: Stephen Kayode S. Ojo, Email: , Email: stephen.ojo@fuoye.edu.ng

Abstract

Introduction: Curative misuse of medicinal plants are worrisome with the paucity of histological information. This led to the investigation of Ipomoea asarifolia in Swiss albino rats infected with Pseudomonas aeruginosa and Staphylococcus aureus.

Methods: Extraction was done using the cold maceration method. The minimum inhibitory concentrations (MIC) of the extracts were determined using the micro-dilution method. Swiss albino rats of 6 sub-groups with 6 animals each (36 animals/organism) were administered with 0.3 ml single oral dose of P. aeruginosa and S. aureus respectively. The animals received treatment for 5 days as follows: 0.5 ml of 5% dimethyl sulphoxide (DMSO) (negative control), 250 mg/kg of amoxicillin (positive control), 2 mg/kg of whole plant extract, 4 mg/kg of whole plant extract, 2 mg/kg of leaf extract, and 4 mg/kg of leaf extract, respectively. The packed cell volume (PCV) and white blood count (WBC) of the animals were determined before and after treatment with histology examination of vital organs.

Results: MIC for S. aureus was 2 mg/mL; the mortality in S. aureus group at 2 mg/kg was 66.7%. The PCV values (50.5±0.5, 45.0±1.0, and 50.5±1.5) decreased after infection, and a corresponding increase in the PCV was observed after treatment with the extracts. Also, a significant increase in the WBC values (3.40±0.35, 4.10±0.15, and 3.30±0.40) following infection and a corresponding decrease after treatment were observed. Congestion of vessels in the kidney was also observed.

Conclusion: I. asarifolia has a dose-dependent antibacterial and curative activity, and could enhance innate immunity.

Keywords: Medicinal plant, Gram negative bacteria, Gram positive bacteria, Antibacterial activity, Animal study, Histology

Implication for health policy/practice/research/medical education: The traditional uses of Ipomoea asarifolia was confirmed as a potential therapeutic agent against commonly isolated hospital acquired infections. Please cite this paper as: Ojo SKS, Sunmonu GT, Adeoye AO, Akinwunmi CF, Obakunle MI, Ojerinde AO, et al. Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus. J Herbmed Pharmacol. 2022;11(3):409-418. doi: 10.34172/jhp.2022.47.
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Submitted: 16 Mar 2022
Revision: 29 Apr 2022
Accepted: 30 Apr 2022
ePublished: 25 Jun 2022
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