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J Herbmed Pharmacol. 2022;11: 339-347.
doi: 10.34172/jhp.2022.39
  Abstract View: 163
  PDF Download: 107

Original Article

Screening of phytochemicals, toxicities, and activities of three Dillenia species

Pornnarong Siripiyasing 1 ORCID logo, Kittiya Silawong 2 ORCID logo, Tikumporn Thooptianrat 3 ORCID logo, Runglawan Sudmoon 4 ORCID logo, Nelly Babayan 5 ORCID logo, Lusine Khondkaryan 5 ORCID logo, Lilit Apresyan 5 ORCID logo, Tawatchai Tanee 6 ORCID logo, Arunrat Chaveerach 3* ORCID logo

1 Faculty of Science and Technology, Rajabhat Maha Sarakham University, Thailand
2 Department in biology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Thailand
3 Department of Biology, Faculty of Science, Khon Kaen University, Thailand
4 Faculty of Law, Khon Kaen University, Thailand
5 Group of Cell Technologies, Institute of Molecular Biology, National Academy of Sciences; Department of Genetics and Cytology, Yerevan State University, Yerevan, RA
6 Faculty of Environment and Resource Studies, Mahasarakham University, Thailand
*Corresponding Author: Email: raccha@kku.ac.th

Abstract

Introduction: Plants containing β-sitosterol and oleamide are important for various diseases. So, Dillenia indica, D. obovata, and D. pentagyna were investigated for phytochemicals, cytotoxicity and genotoxicity levels on peripheral blood mononuclear cells (PBMCs) and Hela cells. The protective effect of D. pentagyna extract on a HepG2 cell line was also investigated.

Methods: Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) were used for phytochemical analysis. 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction (MTT) and comet assays were performed for toxicity testing and protective effects against DNA oxidative damage.

Results: The major components were oleamide and β-sitosterol at 38.464-58.247% and 5.585- 6.887% with concentration and quantity of β-sitosterol at 0.2-0.37 mg/mL and 0.42-0.964 mg/g leaf. The D. indica, D. obovata, and D. pentagyna toxicities on PBMCs showed IC50 values at >430, >430, and 350 µg/mL respectively, with no significant DNA damage (P > 0.05) compared to the negative control group. All plant extracts showed toxic activity on Hela cell with IC50 values at <0.43 µg/mL and induced significant DNA damage (P < 0.05) compared to the negative control group. Conversely, the activity of the D. pentagyna extract indicated low cytotoxic activity against HepG2 (IC50>430 μg/mL), no significant (P > 0.05) DNA damage induction, significantly (P < 0.05) decreased DNA damage level, and tremendous antioxidant effect. Additionally, a combined mixture of all plants in an equal proportion revealed no IC50 value and insignificant DNA damage.

Conclusion: All the studied species contained oleamide and β-sitosterol, with toxicity on Hela cells without toxicity on PBMC. The D. pentagyna species showed high antioxidant effects and no toxicity on HepG2.

Keywords: β-sitosterol, Comet assay, Oleamide, Toxicity

Implication for health policy/practice/research/medical education: This research emphasizes phytochemicals concentrated on oleamide and β-sitosterol, toxicities, and activities of D. indica, D. obovata, and D. pentagyna species. This information can support further uses in human health. Please cite this paper as: Siripiyasing P, Silawong K, Thooptianrat T, Sudmoon R, Babayan N, Khondkaryan L, et al. Screening of phytochemicals, toxicities, and activities of three Dillenia species. J Herbmed Pharmacol. 2022;11(3):339-347. doi: 10.34172/ jhp.2022.39.
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Submitted: 11 Oct 2021
Revision: 06 Mar 2022
Accepted: 02 Apr 2022
ePublished: 25 Jun 2022
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