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J Herbmed Pharmacol. 2021;10(3): 344-350.
doi: 10.34172/jhp.2021.40
  Abstract View: 205
  PDF Download: 246

Original Article

Chemical composition and cytotoxic activity of the essential oil from the aerial parts of Dorema aucheri

Seyed Ahmad Hossiniyan 1 ORCID logo, Farideh ghalamfarsa 1 ORCID logo, Shaghayegh Rostami yasuj 1 ORCID logo, Zeynab salehpour 1 ORCID logo, Mohsen Latifpour 1 ORCID logo, Sajad hassanzadeh 2 ORCID logo, Bahram Mohammadi 1 ORCID logo, Farhad Jadidi-Niaragh 3, 4, 5 ORCID logo, Ali Ganji 6, 7 ORCID logo, Ghasem Ghalamfarsa 1* ORCID logo

1 Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2 Department of Internal Medicine, Yasouj University of Medical Sciences, Yasouj, Iran
3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5 Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
6 Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran
7 Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran

Abstract

Introduction: Herbal products are beneficial compounds with many applications in human life. In this study the chemical composition and cytotoxic activity of the essential oil of the aerial parts of Dorema aucheri were assessed. Methods: The essential oil was extracted by hydrodistillation after drying the aerial parts of D. aucheri, collected from the mountains around Yasuj city in the South-West of Iran. The oil composition was determined by GC/MS. To evaluate in vitro cytotoxic activity, the apoptotic effects of the essential oil were investigated against SW48 and SW1116 colorectal cancer cell lines by (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium) bromide (MTT) assay and flow cytometry. Results: The essential oil yield was obtained 0.02% (W/W). Twenty-five compounds were identified in the oil, and the main constituents were caryophyllene (E) (31.29%), Phytol (14.92%), gurjunene (β-) (9.84%), 3,7,11,15-tetramethyl-2-hexadecen-1-ol (8.7%), and n-hexadecanoic acid (8.09%). The MTT assay showed that the IC50 values of the essential oil for SW48 and SW1116 cell lines were 1.4 and 1.2 mg/mL, respectively. The results of flow cytometry showed that the essential oil significantly increased the apoptosis in SW48 cell line compared with the vincristine (P < 0.05). It also increased the apoptosis in SW1116 cells compared with the vincristine, but this difference is not significant. Conclusion: The essential oil of D. aucheri consisted of high amounts of caryophyllene and showed significant cytotoxic effects against SW48 and SW1116 cancerous cell lines.
Keywords: Apoptosis, Colorectal Cancer, Dorema aucheri, Essential Oil
The essential oil of D. Aucheri comprised of high amounts of caryophyllene and showed significant cytotoxic effects against SW48 and SW1116 cancerous cell lines. Hence, after more comprehensive studies, it might be used as a beneficial herbal source for developing anti-tumor drugs.
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Abstract View: 205

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Submitted: 03 Mar 2021
Revision: 25 May 2021
Accepted: 25 May 2021
ePublished: 02 Jul 2021
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