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J Herbmed Pharmacol. 2021;10(3): 296-303.
doi: 10.34172/jhp.2021.34
  Abstract View: 208
  PDF Download: 200

Original Article

Roselle (Hibiscus sabdariffa L.) calyx water extract ameliorates isoniazid and rifampicin induced liver and renal injuries in rats

Yulia Yusrini Djabir 1* ORCID logo, Jumasni Adnan 2 ORCID logo, Nurul Amalia 2, Nurfadillah Ramli 2, Sartini Sartini 3 ORCID logo, Sukamto Salang Mamada 4 ORCID logo, Usmar Usmar 4 ORCID logo

1 Laboratory of Clinical Pharmacy, Faculty of Pharmacy, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia
2 Faculty of Pharmacy, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia
3 Laboratory of Microbiology, Faculty of Pharmacy, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia
4 Laboratory of Pharmacology and Toxicology, Faculty of Pharmacy, Hasanuddin University, Makassar, South Sulawesi, 90245, Indonesia

Abstract

Introduction: Roselle (Hibiscus sabdariffa L.) calyces possess natural antioxidants that may provide therapeutic benefits. This study aimed to investigate the protective effects of Roselle calyx water extract against isoniazid (INH) and rifampicin (RIF) induced liver and renal toxicities.Methods: Male Wistar rats (150-250 g) were designated into five groups: control group, INH-RIF group that was treated with INH-RIF at the toxic doses (50-100 mg/kg for 4 weeks, followed by 100-200 mg/kg for 2 weeks), and Roselle groups that were treated daily with Roselle extract at the doses 62.5, 125, and 250 mg/kg, respectively prior to INH-RIF administration. Blood samples were withdrawn weekly for 6 weeks before removing rats’ livers and kidneys for tissue malondialdehyde (MDA) and histopathological analysis.Results: The results showed all rats in the INH-RIF group experienced marked elevations of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, creatinine, and tissue MDA levels compared to the controls (P<0.05). In contrast, these biomarkers were maintained at near-normal levels in Roselle extract groups. Significant inflammation and cellular degeneration were found in the liver and renal tissues of the INH-RIF group, which were noticeably reduced with Roselle extract pre-treatment at the dose of 250 mg/kg.Conclusion: It is concluded that Roselle calyx extract can provide protection against liver and renal toxicities induced by INH-RIF administration in rats.
Keywords: Roselle extract, Hibiscus sabdariffa L., Anti-tuberculosis, Hepatotoxicity, Nephrotoxicity
Isoniazid (INH) and rifampicin can induce organ toxicities in tuberculosis patients upon receiving the regimen. The results of this study showed the promising protective effects of Roselle (Hibiscus sabdariffa L.) extract on liver and renal functions and histological structures in an animal model that received INH and rifampicin toxic doses. The results may be translated in clinical application to protect against nephrotoxicity and hepatotoxicity in tuberculosis patients.
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Submitted: 23 Oct 2020
Revision: 07 Dec 2020
Accepted: 22 Jan 2021
ePublished: 01 Jul 2021
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