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J Herbmed Pharmacol. 2021;10(2): 241-248.
doi: 10.34172/jhp.2021.27
  Abstract View: 265
  PDF Download: 38

Original Article

Neuropharmacological studies of ethanolic extract of Vaccinium corymbosum on Alzheimer’s type dementia and catatonia in Swiss albino mice

Hanish Singh Jayasingh Chellammal 1* ORCID logo, Bama VV Menon 2 ORCID logo, Mizaton Hazizul Hasan 1 ORCID logo, Afiq Azil 2 ORCID logo, Muhammad Taufiq Suhaimi 2 ORCID logo, Pavithiraa Chandarasekaran 2 ORCID logo, Yasothini Murugan 2 ORCID logo

1 Department of Pharmaceutical Pharmacology and Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia
2 School of Pharmacy, KPJ Healthcare University College, Kota Seriemas, Nilai, Negeri Sembilan, 71800, Malaysia

Abstract

Introduction: Neuroactive herbal drugs enriched with antioxidants are valuable in treating neurocognitive dysfunction and Vaccinium corymbosum, enriched with antioxidant phytochemicals, is used for treating memory disorders. Hence, the present study evaluated the neuroprotective effects of ethanolic extract of Vaccinium corymbosum (EEVC) on aluminium chloride(AlCl3)-induced Alzheimer’s type of dementia and haloperidol-induced catalepsy-associated behavioural changes.Methods: In vitro antioxidant potential was evaluated using 1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The total phenolic content (TPC) was quantified. For in vivo studies, AlCl3 (100 mg/kg) was orally administered for 42 days, whereas the EEVC was administered on the 21st day until the 42nd day in two doses (200 mg/kg and 400 mg/kg). In the haloperidol-induced group, EEVC was treated for 21 days, and haloperidol (1 mg/kg) was administered to induce behavioural changes. Open-field, Y-Maze and traction tests were performed, and the mice brain acetylcholinesterase (AChE) enzyme was determined. Results: IC50 values in DPPH and ABTS assays were 85.5 μg/mL and 80 μg/mL, respectively and the total phenolic content of EEVC was found to be 0.166 mg. In a behavioral study, animals treated with 200 mg/kg and 400 mg/kg of EEVC exhibited a neuroprotective impact on AlCl3-induced neurodegeneration and haloperidol-induced behavioral changes with significant inhibition (P < 0.05 and P < 0.01, respectively) in acetylcholinesterase enzyme. Conclusion: The neuroprotection by EEVC postulated that it is a promising therapeutic agent for treating behavioral and cognitive dysfunctions. Further investigations on pro-inflammatory cytokine and neuroendocrine regulation in transgenic Alzheimer’s disease (AD)models complement the therapeutic value of V. corymbosum.
Keywords: Vaccinium corymbosum, Neurodegeneration, Behavioural changes, Alzheimer's disease, Aluminium, Neurotoxicity
The neuroactive effect of Vaccinium corymbosum could be seen in AlCl3-induced neurodegeneration and haloperidol-induced behavioral changes. The potential antioxidant property and acetylcholinesterase-inhibitory effects of the ethanolic extracts of V. corymbosum played a pivotal role in the attenuation of behavioral changes and remarkably improved learning and memory. Hence, it might be beneficial in patients with Alzheimer’s disease.
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Abstract View: 265

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Submitted: 15 Aug 2020
Revision: 13 Oct 2020
Accepted: 26 Oct 2020
ePublished: 07 Jan 2021
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