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J Herbmed Pharmacol. 2020;9(3): 286-292.
doi: 10.34172/jhp.2020.36
  Abstract View: 118
  PDF Download: 68

Original Article

Assessment of the anti-diabetic potential of the Cratoxylum formosum subsp. formosum extracts via carbohydrate hydrolyzing enzymes inhibitory activities

Kornkanok Arsakit 1 ORCID logo, Benchaluk Thongchuai 1 ORCID logo, Sutthira Sedlak 2 ORCID logo, Serm Surapinit 1* ORCID logo

1 Department of Medical Technology, School of Allied Health Sciences, University of Phayao, Phayao 56000, Thailand
2 Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, Thailand
*Corresponding author: Serm Surapinit, Email: serm.su@up.ac.th

Abstract

Introduction: This research aims to evaluate the anti-diabetic activity of the extracts from different parts of Cratoxylum formosum subsp. formosum.

Methods: The in vitro inhibitory activities of the hexane (HEX), dichloromethane (DCM) and ethyl acetate (EtOAc) extracts from the flowers, leaves, roots and stems on pancreatic α-amylase (pAA), Saccharomyces α-glucosidase (SAG), rat intestinal maltase (rIM), and sucrase (rIS) were investigated.

Results: The DCM and EtOAc extracts from the flowers (IC50 5.4 ± 1.5 and 10.5 ± 0.6 µg/mL) displayed the similar inhibitory activities as acarbose (IC50 7.2 ± 0.4 µg/mL) in the pAA assay. The inhibitory activities of the DCM and EtOAc extracts from the flowers (IC50 56.7 ± 8.9 and 20.4 ± 0.4 µg/mL), EtOAc extract from leaves (IC50 45.0 ± 3.5 µg/mL), DCM and EtOAc extracts from roots (IC50 35.0 ± 6.7 and 16.7± 3.6 µg/mL), and EtOAc extract from stems (IC50 31.1 ± 7.3 µg/mL) were more potent than acarbose (IC50 431.4 ± 16.7 µg/mL) on SAG inhibitory assay (P<0.05). In the rIM assay, DCM and EtOAc extracts from the flowers (IC50 8.5 ± 0.2 and 12.4 ± 0.3 µg/mL) exhibited stronger inhibitory activity than acarbose (IC50 38.5 ± 7.2 µg/mL) (P<0.05). Moreover, the inhibitory activity of DCM extract from the flowers (IC50 16.9 ± 1.5 µg/ mL) was comparable to the acarbose (IC50 15.5 ± 1.2 µg/mL) on rIS assay.

Conclusion: The DCM and EtOAc extracts from the flowers were more active than the leaves, roots and stems in the inhibition of our defined target enzymes.

Keywords: Cratoxylum, Medicinal plants, Alpha-amylase inhibitor, Hypoglycemic Agents

Implication for health policy/practice/research/medical education:

The flower of C. formosum subsp. formosum is the edible part and exert good carbohydrate hydrolyzing enzymes inhibitory activities. This part of this plant plausibly applied as a functional food to prevent and treat diabetes.

Please cite this paper as: Arsakit K, Thongchuai B, Sedlak S, Surapinit S. Assessment of the anti-diabetic potential of the Cratoxylum formosum subsp. formosum extracts via carbohydrate hydrolyzing enzymes inhibitory activities. J Herbmed Pharmacol. 2020;9(3):286-292. doi: 10.34172/jhp.2020.36.

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Submitted: 29 Jan 2020
Accepted: 06 Apr 2020
ePublished: 02 May 2020
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