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J Herbmed Pharmacol. 2020;9(3): 268-276.
doi: 10.34172/jhp.2020.34
  Abstract View: 123
  PDF Download: 68

Original Article

Ultra performance liquid chromatography-tandem mass spectrometeric analysis of ethyl acetate fraction from saudi Lavandula coronopifolia Poir and evaluation of its cytotoxic and antioxidant activities

Sahar Abdelaziz 1 ORCID logo, Wafaa H. B. Hassan 1 ORCID logo, Ahmed E. M. Elhassanny 2* ORCID logo, Hanan M. Al-Yousef 3 ORCID logo, May A. Elsayed 1 ORCID logo, Rasha Adel 1 ORCID logo

1 Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Egypt
2 Department of Pharmacology and Toxicology, Brody School of Medicine, East Carolina University, Greenville, North Carolina, 27834, USA
3 Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
*Corresponding author: Ahmed E. M. Elhassanny, Tel: +1 2524125335, Email: elhassannya16@ecu.edu

Abstract

Introduction: The ethyl acetate fraction of the Saudi Lavandula coronopifolia Poir has been previously reported to have hepatoprotective activity against ethanol-induced oxidative stress. The aim of the current study was to investigate the chemical composition, cytotoxic effect, and antioxidant activities of ethyl acetate fraction of the aerial parts of Saudi L. coronopifolia Poir.

Methods: Air dried aerial parts of L. coronopifolia were extracted using 90% ethyl alcohol. The dried extract was suspended in water, defatted with light petroleum and then fractionated with ethyl acetate. The ethyl acetate fraction was subjected to ultra performance liquid chromatography-tandem mass spectrometeric (UPLC-ESI/MS/MS) analysis in a negative ionization mode. The antioxidant activity of the fraction was determined using free radical 2,2-diphyenyl-picrylhydrazyl (DPPH) scavenging assay and its cytotoxic effect against HepG2 (human hepatocarcinoma) and MCF-7 (human breast carcinoma) cells were determined using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) cell viability assay.

Results: The major components of the ethyl acetate fraction included carvacrol-O-diglucoside, (34.98%) and trihydroxy ursolic acid (12.07%). Moreover, the DPPH radical scavenging activity of ethyl acetate fraction was measured. The ethyl acetate fraction revealed an antioxidant potential with EC50 17.8 ± 1.3 µg/mL. Additionally, he ethyl acetate fraction showed cytotoxic activity against HepG-2 and MCF-7 cells with IC50=29.3 ± 0.9 µg/mL and 14.6 ± 0.3 µg/mL, respectively.

Conclusion: The ethyl acetate fraction of the Saudi L. coronopifolia has antioxidant activity and also cytotoxic activity against breast and liver cancer cells.

Keywords: Lavandula coronopifolia Poir, UPLC-ESI-MS/MS, Antioxidant, Cytotoxicity

Implication for health policy/practice/research/medical education:

The ethyl acetate fraction of Lavandula coronopifolia is an affordable source for compounds which have antioxidant activity and also cytotoxic activity againt heaptic and breast cancers.

Please cite this paper as: Abdelaziz S, Hassan WHB, Elhassanny AEM, Al-Yousef HM, Elsayed MA, Adel R. Ultra performance liquid chromatography-tandem mass spectrometeric analysis of ethyl acetate fraction from saudi Lavandula coronopifolia Poir and evaluation of its cytotoxic and antioxidant activities. J Herbmed Pharmacol. 2020;9(3):268-276. doi: 10.34172/jhp.2020.34.

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Submitted: 31 Dec 2019
Accepted: 21 Feb 2020
ePublished: 02 May 2020
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