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J Herbmed Pharmacol. 2020;9(1): 81-85.
doi: 10.15171/jhp.2020.12
  Abstract View: 765
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Short Communication

Phytochemical analysis and anti-inflammatory activity of Cladophora aegagropila extract

Aleksandra Aleksandrova 1 ORCID logo, Mariia Nesterkina 1* ORCID logo, Svitlana Gvozdii 2 ORCID logo, Iryna Kravchenko 1 ORCID logo

1 Department of Organic and Pharmaceutical Technologies of Odessa National Polytechnic University, Odessa, Ukraine
2 Department of Human Health and Civil Safety, I.I. Mechnikov Odessa National University, Odessa, Ukraine
*Corresponding author: Mariia Nesterkina, Email: mashaneutron@gmail.com

Abstract

Introduction: The aim of this research is centered on phytochemical analysis and anti-inflammatory activity of Cladophora aegagropila extract.

Methods: Total flavonoid content in the appropriate ethanol extract of C. aegagropila was determined using the spectrometric method. Anti-inflammatory activity was evaluated by the models of carrageenan-induced and allyl isothiocyanate-induced (AITC-induced) inflammation of hind limb in rats. Experimental animals were divided into 3 groups, 5 animals each: 1st group – control; the animals without therapy of induced inflammation, 2nd group – animals were treated with application of 5% ointment containing C. aegagropila; 3rd group – animals were treated with application of 5% ibuprofen. Evaluation of anti-inflammatory activity was performed by determination of dynamic change of thickness and volume of affected animal limb for carrageenan-induced inflammation for 8 days (with daily registration of records), and for AITC induced inflammation for 24 h (with registration of records after 30 min, 1 h, 2 h, 3 h, 6 h and 24 h since phlogogen was introduced).

Results: Flavonoids content in C. aegagropila extract varied depending on ethanol concentration: usage of 40% ethanol led to 18.2 mg of flavonoid recovery on 1.0 g of dry raw material; 70% ethanol – 39.5 mg and 96% ethanol – 35.5 mg. Therapy of 5% ointment based on C. aegagropila extract decreased inflammatory response caused by the subplantar introduction of the corresponding phlogogen.

Conclusion: The ointment from C. aegagropila extract shows anti-inflammatory activity by inhibiting inflammation caused by AITC and carrageenan.

Keywords: Cladophora aegagropila, Phytochemical analysis, Anti-inflammatory activity

Implication for health policy/practice/research/medical education:

Cladophora aegagropila extract possesses significant anti-inflammatory action and might be used as an effective natural agent for inflammation treatment.

Please cite this paper as: Aleksandrova A, Nesterkina M, Gvozdii S, Kravchenko I. Phytochemical analysis and antiinflammatory activity of Cladophora aegagropila extract. J Herbmed Pharmacol. 2020;9(1):81-85. doi: 10.15171/jhp.2020.12.

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Submitted: 19 Apr 2019
Accepted: 17 Jul 2019
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