J Herbmed Pharmacol. 2020;9(1):35-41.
doi: 10.15171/jhp.2020.05
  Abstract View: 37
  PDF Download: 45

Original Article

Effect of Matricaria chamomilla hydro-alcoholic and flavonoids rich extracts on rat isolated uterus

Hassan Sadraei 1 * ORCiD, Seyed Ebrahim Sajjadi 2, Gholamreza Asghari 2, Majid Khalili 1

1 Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Pharmacognosy and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
*Corresponding author: Hassan Sadraei, Email: Email: sadraei@pharm.mui.ac.ir

Abstract

Introduction: Pharmacological studies confirm antispasmodic activities of chamomile (Matricaria chamomilla) extract on intestinal smooth muscles and it has been suggested that chamomile increases uterus tone, but so far there is no scientific studies which support this assumption. Therefore, this study was designed to determine spasmodic and spasmolytic activities of M. chamomilla extracts on rat isolated uterus.

Methods: Hydro-alcoholic extract of M. chamomilla was prepared by maceration technique. Flavonoids rich extract was prepared by liquid in liquid extraction technique. The spasmodic effects of the extracts were assessed on spontaneously contracting rat uterus. The myorelaxant effect of M. chamomilla extracts was validated on isolated uterus contractions induced by KCl, acetylcholine (ACh), electrical field stimulation (EFS) and oxytocin.

Results: Hydro-alcoholic extract of M. chamomilla (0.8 and 1.6 mg/mL) enhanced spontaneous movement of rat isolated uterus smooth muscle suspended in an organ bath. On the other hand, flavonoids rich fraction only diminished uterus contractile activities. Flavonoids rich extract of the plant at bath concentration ranges of 40 μg/mL to 400 μg/mL attenuated uterus response to ACh, KCl, EFS and oxytocin. The hydro-alcoholic extract of M. chamomilla at higher concentration ranges (250 μg/mL to 1.5 mg/mL) inhibited uterus contractions induced by the above spasmogens.

Conclusion: The present study confirms both spasmodic and spasmolytic activities M. chamomilla hydro-alcoholic extract. Therefore, medicinal use of the crude extract of M. chamomilla may initiate uterus contraction which could increase risk of spontaneous miscarriage or premature parturition.

Implication for health policy/practice/research/medical education:

This paper provides pharmacological evidence for spasmogenic and spasmolytic actions of M. chamomilla extract on isolated uterus. As M. chamomilla potentiated irregular periodic uterus contraction it should not be used during pregnancy.

Please cite this paper as: Sadraei H, Sajjadi SE, Asghari G, Khalili M. Effect of Matricaria chamomilla hydro-alcoholic and flavonoids rich extracts on rat isolated uterus. J Herbmed Pharmacol. 2020;9(1):35-41. doi: 10.15171/jhp.2020.05.

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Submitted: 30 Apr 2019
Accepted: 01 Oct 2019
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