J Herbmed Pharmacol. 2019;8(4):308-313.
doi: 10.15171/jhp.2019.45
  Abstract View: 175
  PDF Download: 159

Original Article

The impact of caffeic acid phenethyl ester, chrysin and ethanolic extracts of propolis on PLD1 gene expression in AGS cell line

Hamideh Jafari-Ghahfarokhi 1 ORCiD, Ali Jazaeri 1, Nematallah Amini-Sarteshnizi 2, Hossein Teimori 1 * ORCiD

1 Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Introduction: Up-regulation of phospholipase D (PLD) is functionally related to tumorigenesis and oncogenic signals. Chrysin, caffeic acid phenethyl ester (CAPE) and ethanolic extract of propolis (EEP) are three safe compounds which have been shown to possess antiproliferative, antioxidant, anti-inflammatory and antineoplastic properties. In this study, the effects of these three compounds on PLD1 gene expression were examined in AGS cell line.

Methods: After determining the appropriate concentrations of EEP, CAPE, and chrysin, AGS cells were cultured in mediums with proper ratios of the compounds. AGS cells were maintained in exponential growth and the culture mediums refreshed every other day. Finally, after extracting total RNA from AGS cells, real-time PCR was performed to evaluate the mRNA expression of PLD1 in the presence of each compound.

Results: CAPE decreased the mRNA level of PLD1 gene in a dose-dependent manner. A solution with 30 μM concentration of CAPE was the effective dose in comparison to control group as well as 15 and 20 μM concentrations of the compound, whereas no changes were observed in the presence of EEP and chrysin.

Conclusion: Taken together, the results of the study indicated that CAPE might exert its anti-neoplastic effect by targeting PLD1 expression in AGS cell line.

Implication for health policy/practice/research/medical education:

Caffeic acid phenethyl ester (CAPE) can effectively inhibit cell proliferation in gastric cancer cell line and might be useful in the treatment of patients with gastric cancer.

Please cite this paper as: Jafari-Ghahfarokhi H, Jazaeri A, Amini-Sarteshnizi N, Teimori H. The impact of caffeic acid phenethyl ester, chrysin and ethanolic extracts of propolis on PLD1 gene expression in AGS cell line. J Herbmed Pharmacol. 2019;8(4):308- 313. doi: 10.15171/jhp.2019.45.

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Submitted: 27 Apr 2019
Accepted: 07 Jun 2019
First published online: 04 Sep 2019
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