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J Herbmed Pharmacol. 2020;9(2): 121-129.
doi: 10.34172/jhp.2020.16
  Abstract View: 179

Original Article

Iranian brown propolis possesses neuroprotective effect against ischemic neuronal damage in mice

Gholamreza Bazmandegan 1, 2 ORCID logo, Ali Shamsizadeh 1, 3 ORCID logo, Mohsen FathiNajafi 4 ORCID logo, Zahra Assadollahi 5 ORCID logo, Mohammad Allahtavakoli 1, 3 ORCID logo, Zahra Kamiab 2, 6 ORCID logo, Alireza Vakilian 7, 8 ORCID logo, Amir Moghadam Ahmadi 7, 8 ORCID logo, Morteza Amirteimoury 9 ORCID logo, Mohammad Taher Boroushaki 10 * ORCID logo

1 Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2 Clinical Research Development Unit, Ali-Ibn Abi-Talib Hospital, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
3 Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
4 Razi Vaccine and Serum Research Institute, Mashhad Branch, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran
5 Department of Epidemiology and Biostatistics, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
6 Department of Family Medicine, Ali-Ibn Abi-Talib Hospital , School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
7 Non-Communicable Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
8 Department of Neurology, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
9 Student Research Committee, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
10 Pharmacological Research Center of Medicinal Plants, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
*Corresponding author: Mohammad Taher Boroushaki, Email: BoroushakiMT@mums.ac.ir

Abstract

Introduction: Stroke is one of the leading causes of death and disability worldwide. Propolis, a polyphenol-rich resinous product processed by honeybees from a variety of plant sources, has a set of biological activities. We investigated the neuroprotective effect of Iranian brown propolis (IBP) in a mouse model of permanent middle cerebral artery occlusion (MCAO).Methods: Experimentally, water extracts of propolis (WEPs) were obtained from Kerman (KeWEP) and Khorasan Razavi (KhWEP) provinces, Iran. The chemical characterization and total polyphenol content of WEPs were determined using the Folin–Ciocalteu assay and gas chromatography-mass spectrometry (GC-MS). Animals were divided into eight experimental groups including: sham, control, and three groups each of which KeWEP- and KhWEP-treated mice. The drugs were administered at doses of 30, 100 and 200 mg/kg, intraperitoneally (IP), during four different time points. Infarct volume and brain edema were measured at 48 h. Behavioral tests were evaluated at 4, 24 and 48-hour post stroke.Results: The total polyphenol content was 1100 and 1400 mg/L in KhWEP and KeWEP respectively. Compared to the control group, the doses of 100 and 200 mg/kg in both samples decreased infarct volume. Brain edema was also reduced in all treatment groups. The dose of 200 mg/kg in both samples and 100 mg/kg in the KeWEP-treated group significantly increased grasping ability. Sensory-motor function was improved in all groups, too.Conclusion: These results suggest that IBP may reduce ischemic brain injury by its neuroprotective effect on focal cerebral ischemia.
Keywords: Cerebral ischemia, Brown propolis, Neuroprotection, Polyphenol
This paper provides pharmacological evidence for neuroprotective effect of Iranian brown propolis in a mouse model of permanent middle cerebral artery occlusion.
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Submitted: 20 May 2019
Accepted: 19 Aug 2019
ePublished: 14 Feb 2020
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