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J Herbmed Pharmacol. 2015;4(2): 49-52.
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Original Article

Cytotoxic activities of Euphorbia kopetdaghi against OVCAR-3 and EJ-138 cell lines

Mahmoud Aghaei 1, Mustafa Ghanadian 2,3*, Farough Faez 3, Ebrahim Esfandiary 4

1 Department of Biochemistry, Isfahan Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Pharmacognosy, Isfahan Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran
*Corresponding author: Mustafa Ghanadian, Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran. Tel: +98-3137922553, ghannadian@gmail.com

Abstract

Introduction: Over the centuries, the genus Euphorbia was known to be toxic to humans and animals. Recently, in a primary study significant suppressive activity against phytohemagglutinin activated T-cell proliferation has been reported from this plant. Therefore, this study was designed to evaluate the cytotoxic effects of different parts of E. kopetdaghi against cancer cell lines.

Methods: Filtration and in vacuo concentration resulted in a green gum which was subjected on silica gel CC (hexane/Acetone, 0 →50) to several fractions: F1-F8. The inhibitory effects of obtained fractions with 5, 50, and 500 μg/ml concentrations were evaluated on proliferation and viability of cancer cells (OVCAR and EJ-138) in 48 hours treatment. Finally, cell viability was determined at a wavelength of 570 by 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method.

Results: Based on studies of microscopic observation and viability testing, F1, F2, F4, F5, F6, and F7 showed significant cytotoxic effect at concentration of 50 and 500 μg/ml against EJ-138 and OVCAR-3 cell lines. These fractions inhibited growth of EJ-138 and OVCAR-3 cells in a concentration-dependent manner. Fraction of F8 induced tumor promotion significantly in EJ-138 and OVCAR-3 cells, respectively.

Conclusion: Due to the inhibitory properties of E. kopetdaghi extract and its fractions on cancer cells of OVCAR3 and EJ-13, isolation, purification and identification of compounds presented in the fractions possessing cytotoxic effects are recommended which were the area of our future research.

Keywords: Euphorbiaceae, Euphorbia kopetdaghi, cytotoxicity, OVCAR

Implication for health policy/practice/research/medical education:

In the present study, we evaluated the cytotoxicity properties of E. kopetdaghi extract and its fractions on cancer cells of OVCAR3 and EJ-138. The observed moderated toxicity of fractions indicated that this plant has the value of more phytochemical consideration to find possible anti-cancer lead compounds.

Please cite this paper as: Aghaei M, Ghanadian M, Faez F, Esfandiary E. Cytotoxic activities of Euphorbia kopetdaghi against OVCAR-3 and EJ-138 cell lines. J Herbmed Pharmacol 2015; 4(2): 49-52.

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