J Herbmed Pharmacol. 2018;7(2):100-105.
doi: 10.15171/jhp.2018.17

Scopus id: 85045020026
  Abstract View: 377
  PDF Download: 323

Original Article

Antispasmodic activity of apigenin and luteolin, two components of Dracocephalum kotschyi extract, on rat ileum contractions

Hassan Sadraei 1 * ORCiD, Mostafa Ghanadian 2, Golamreza Asghari 2, Nastaran Sekhavati 1, 2

1 Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Introduction: Aerial parts of Dracocephalum kotschyi have been used as antispasmodic agents in Iranian traditional medicine. Recent pharmacological studies confirmed antispasmodic activity of D. kotschyi extract. The objective of this research was to investigate antispasmodic activities of apigenin and luteolin to find out if they are responsible for the spasmolytic activity of hydroalcoholic extract of D. kotschyi. Methods: Aerial parts of D. kotschyi were extracted with ethanol. Antispasmodic effect of hydroalcoholic extract of D. kotschyi, apigenin and luteolin were examined on KCl and/or acetylcholine (ACh)-induced contractions in rat isolated ileum. Results: Hydroalcoholic extract of D. kotschyi concentrations-dependently inhibited KCl and ACh induced contractions with IC50 values of 41 ± 10 μg/mL and 133 ± 19 μg/mL, respectively. Apigenin concentrations-dependently inhibited KCl and ACh induced contractions with IC50 values of 57 ± 12 μM and 80 ± 18 μM, respectively. Luteolin concentrations-dependently inhibited KCl induced contractions with IC50 values of 68 ± 14 μM. Loperamide reduced both KCl and ACh induced contraction with IC50 values of 189 ± 44 nM and 82 ± 20 μM, respectively. Conclusion: In this study apigenin and luteolin were identified as two active ingredients responsible for antispasmodic activities of D. kotschyi extract.
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Submitted: 14 Jun 2017
Revised: 13 Aug 2017
Accepted: 20 Mar 2018
First published online: 20 Mar 2018
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