Shahrekord University of Medical Sciences Journal of Herbmed Pharmacology 2345-5004 15 4 2026 10 01 Propolis versus chlorhexidine mouthwash following impacted mandibular third molar surgery: A randomized clinical trial 458 467 10.34172/jhp.54044 EN Amir Najafi https://orcid.org/0000-0002-6840-0751 Fatemeh Shirvani https://orcid.org/0009-0004-2792-8949 Nazli Rabienejad https://orcid.org/0000-0003-2176-9786 Samad Moslehi https://orcid.org/0000-0003-1597-7327 Negin Najafi https://orcid.org/0000-0003-3708-7982 Journal Article 10.34172/jhp.54044 2026 07 17 2026 09 14 Introduction: Propolis has anti-inflammatory, antimicrobial, and wound-healing properties. This study compared the effects of propolis and chlorhexidine mouthwash on the gingival index (GI), plaque index (PI), and incidence of dry socket (DS) after surgical extraction of impacted lower third molars. Methods: This randomized, double-blind, parallel-group clinical trial included 32 patients aged 15-30 years undergoing surgical extraction of an impacted mandibular third molar. Patients were randomly allocated to receive either a propolis mouthwash (test group) or 0.2% chlorhexidine mouthwash (control group). The primary outcome was GI severity. Secondary outcomes included PI and DS severity, which were evaluated at baseline, day 7, and day 14. Statistical analyses were performed using SPSS version 26 by Fisher’s exact test, the chi-Square test, and an ordinal mixed-effects model to analyze categorical variables. Results: A total of 32 participants, with 16 in each group, completed the study. Generalized linear mixed model analysis indicated no statistically significant between-group differences in GI (P = 0.783) or PI (P = 0.793) over the 14-day follow-up. Similarly, DS severity showed no drug difference (P = 0.906), although both groups improved significantly over time (P = 0.045). Notably, tooth discoloration was descriptively more frequent and severe in the chlorhexidine group, whereas no severe discoloration occurred in the propolis group; however, the difference was not statistically significant (P = 0.185). Conclusion: Propolis mouthwash demonstrated comparable clinical outcomes to chlorhexidine in reducing gingival inflammation, plaque accumulation, and DS incidence, with a potentially lower incidence of side effects. However, more studies with larger sample sizes are required to confirm the results.