Shahrekord University of Medical Sciences Journal of Herbmed Pharmacology 2345-5004 12 4 2023 09 01 Phytochemical identification and in silico study of ethanolic extract of white cabbage as a phosphodiesterase 1B inhibitor 521 535 10.34172/jhp.2023.45004 EN Nazir Ahmad https://orcid.org/0000-0001-9948-7653 Kaisun Nesa Lesa https://orcid.org/0000-0003-1001-0221 Navista Sri Octa Ujiantari https://orcid.org/0000-0001-6398-704X Ari Sudarmanto https://orcid.org/0000-0003-0991-4311 Zullies Ikawati https://orcid.org/0000-0002-4812-055X Nanang Fakhrudin https://orcid.org/0000-0001-7954-9503 Journal Article 10.34172/jhp.2023.45004 2023 04 15 2023 06 14 Introduction: Memory dysfunction has remained a challenging issue globally. Nootropics have proven fruitful in managing cognitive dysfunction but because of their side effects, opportunities exist to explore alternatives. White cabbage is a cost-effective natural source of phytochemicals without side effects and has remained uninvestigated as a nootropic agent. This study sought to identify secondary metabolites in white cabbage extract (WCE) and to predict the molecular interaction between the phytochemical constituents of cabbage and phosphodiesterase-1B (PDE1B) using in silico studies. Methods: The WCE was prepared by macerating crushed fresh white cabbage with ethanol for 24 h with continuous stirring. The phytochemical profile of WCE was analyzed using thin layer chromatography (TLC)-densitometry, and molecular docking studies were performed to predict the underlying mechanism action of the phytochemicals with PDE1B. Results: The TLC-densitometry analysis showed that WCE was a rich source of sinigrin, whereas quercetin, chlorogenic acid, and rutin were not detected. In silico studies identified neobrassicin as having the highest affinity (∆Gbind: −19.3358 kcal/mol) for PDE1B. However, quercetin (∆Gbind: −13.1813 kcal/mol) and chlorogenic acid (∆Gbind: −14.8706 kcal/mol) exhibited moderate interaction with PDE1B. Conclusion: These results suggest that WCE has the potency to improve memory function by blocking PDE1B, and this preliminary study implies upcoming in vitro and in vivo research.