Shahrekord University of Medical Sciences Journal of Herbmed Pharmacology 2345-5004 9 1 2020 01 01 Phytochemical analysis and anti-inflammatory activity of Cladophora aegagropila extract 81 85 10.15171/jhp.2020.12 EN Aleksandra Aleksandrova https://orcid.org/0000-0002-5930-6843 Mariia Nesterkina https://orcid.org/0000-0002-3201-7961 Svitlana Gvozdii https://orcid.org/0000-0002-8208-2972 Iryna Kravchenko https://orcid.org/0000-0001-8929-8701 Journal Article 10.15171/jhp.2020.12 2019 04 19 2019 07 17 Introduction: The aim of this research is centered on phytochemical analysis and anti-inflammatory activity of Cladophora aegagropila extract. Methods: Total flavonoid content in the appropriate ethanol extract of C. aegagropila was determined using the spectrometric method. Anti-inflammatory activity was evaluated by the models of carrageenan-induced and allyl isothiocyanate-induced (AITC-induced) inflammation of hind limb in rats. Experimental animals were divided into 3 groups, 5 animals each: 1st group – control; the animals without therapy of induced inflammation, 2nd group – animals were treated with application of 5% ointment containing C. aegagropila; 3rd group – animals were treated with application of 5% ibuprofen. Evaluation of anti-inflammatory activity was performed by determination of dynamic change of thickness and volume of affected animal limb for carrageenan-induced inflammation for 8 days (with daily registration of records), and for AITC induced inflammation for 24 h (with registration of records after 30 min, 1 h, 2 h, 3 h, 6 h and 24 h since phlogogen was introduced). Results: Flavonoids content in C. aegagropila extract varied depending on ethanol concentration: usage of 40% ethanol led to 18.2 mg of flavonoid recovery on 1.0 g of dry raw material; 70% ethanol – 39.5 mg and 96% ethanol – 35.5 mg. Therapy of 5% ointment based on C. aegagropila extract decreased inflammatory response caused by the subplantar introduction of the corresponding phlogogen. Conclusion: The ointment from C. aegagropila extract shows anti-inflammatory activity by inhibiting inflammation caused by AITC and carrageenan.