Shahrekord University of Medical Sciences Journal of Herbmed Pharmacology 2345-5004 4 1 2015 01 01 Histomorphometric study on the effects of Artemisia sieberi extract in mice skin 20 24 EN Jahangir Kaboutari Mohammad Saeed Haydarnejad Rahmat allah Fatahian Dehkordi Sedigheh Raeisi Vanani Journal Article Introduction: Skin as the biggest single body organ is always exposing to various injuries, therefore health and healing of its injuries is vital. Artemisia sieberi is a valuable medicinal plant with a long history of indication in folk and modern medicine. Due to different chemical components and antioxidant, anti-inflammatory, antimicrobial and cytoprotective properties of Artemisia, this study was conducted to study the histomorphometric effects of Artemisia sieberi (A. sieberi) extract on mice skin. Methods: Ninety adult mice were randomly divided in 3 groups. In the treatment group A. sieberi extract dissolved in ethanol & distilled water, in the positive control ethanol & distilled water, and in negative control only distilled water were applied on the shaved dorsum twice daily for 21 days. Mean thickness of epidermis, hypodermis & dermis layers, percentage of collagen fibers and histological evaluation of skin layers were studied in 1, 3, 5, 14 and 21days post treatment. Data were presented as mean± SD and analyzed using one way ANOVA and LSD post hoc tests. The P<0.05 was considered as statistically significant. Results: A. sieberi extract significantly increased epidermis thickness in day 1, hypodermis, dermis and percentage of collagen fibers in day 3 in comparison to positive and negative control groups. Histology study revealed normal structure of skin and no abnormality was seen. Conclusion: A. sieberi extract can be effective for health and healing of skin injuries by increasing thickness of the skin layers and amount of collagen fibers.