J Herbmed Pharmacol. 2019;8(4):333-338.
doi: 10.15171/jhp.2019.49
  Abstract View: 53
  PDF Download: 40

Short Communication

Potentiation of in vitro apoptotic effects of δ-Tocotrienol and Jerantinine A on human lung adenocarcinoma cells

Ibrahim Babangida Abubakar 1, 2 * ORCiD, Hwei-San Loh 1, 3 ORCiD

1 School of Biosciences, Faculty of Science, The University of Nottingham Malaysia Campus, Jalan Broga 43500 Semenyih, Selangor Darul Ehsan, Malaysia
2 Department of Biochemistry, Kebbi State University of Science and Technology, PMB 1144, Alero, Kebbi State, Nigeria
3 Biotechnology Research Centre, The University of Nottingham Malaysia Campus, Jalan Broga 43500 Semenyih, Selangor Darul Ehsan, Malaysia

Abstract

Introduction: The apoptotic effects of single-compound and combined sub-effective concentrations of δ-tocotrienol and jerantinine A on human lung adenocarcinoma (A549) cells were investigated.Methods: Assays including cell viability, histochemical and immunofluorescence staining techniques, flow cytometry and enzyme activity were used.Results: The combination of δ-tocotrienol with jerantinine A at sub-effective concentrations induced a synergistic effect and improved selective toxicity towards cancerous A549 cells over normal lung fibroblast (MRC5) cells compared to the single-compound jerantinine. Morphological features of apoptosis were evident on treated A549 cells. Combined sub-effective concentrations of δ-tocotrienol with jerantinine A induced a predominantly G2/M cell cycle arrest and characterised by a disruption of microtubular networks mediated via caspase 8, 9 and 3 enzymatic activities.Conclusion: These findings demonstrated improved potency in vitro and reduced dose-related toxicity of jerantinine A to normal cells through prospective combined treatment between low-concentration δ-tocotrienol and jerantinine A for lung cancer.
Introduction: The apoptotic effects of single-compound and combined sub-effective concentrations of δ-tocotrienol and jerantinine A on human lung adenocarcinoma (A549) cells were investigated.Methods: Assays including cell viability, histochemical and immunofluorescence staining techniques, flow cytometry and enzyme activity were used.Results: The combination of δ-tocotrienol with jerantinine A at sub-effective concentrations induced a synergistic effect and improved selective toxicity towards cancerous A549 cells over normal lung fibroblast (MRC5) cells compared to the single-compound jerantinine. Morphological features of apoptosis were evident in treated A549 cells. Combined sub-effective concentrations of δ-tocotrienol with jerantinine A induced a predominantly G2/M cell cycle arrest and characterized by a disruption of microtubular networks mediated via caspase 8, 9 and 3 enzymatic activities.Conclusion: These findings demonstrated improved potency in vitro and reduced dose-related toxicity of jerantinine A to normal cells through prospective combined treatment between low-concentration δ-tocotrienol and jerantinine A for lung cancer.
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Submitted: 28 Apr 2019
Revised: 12 May 2019
Accepted: 08 Jun 2019
First published online: 04 Sep 2019
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