Evaluation of the effect of Chrysin and Caffeic acid phenethyl ester on eIF4E expression in AGS cell line

Marziyeh Abolhasani; Morteza Hashemzadeh-Chaleshtori; Abbas Doosti; Nematolah Amini  Sarteshnizi; Mostafa Gholami-Arjenaki; Hossein Teimori

J Herbmed Pharmacol. 2014;3(2): 129-133.

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Original Article

Evaluation of the effect of Chrysin and Caffeic acid phenethyl ester on eIF4E expression in AGS cell line

Marziyeh Abolhasani ¹, Morteza Hashemzadeh-Chaleshtori ², Abbas Doosti ¹, Nematolah Amini Sarteshnizi, Mostafa Gholami-Arjenaki ⁴, Hossein Teimori ²^*

¹ Department of Biology, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
² Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
³ Department of Biology, University of Mohaghegh Ardabili, Ardabil, Iran
⁴ Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

*Corresponding Author: *Corresponding author: Hossein Teimori, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. Tel.: +98-381-3346692, Fax: +98-381-3330709,, Email: hosseintimm@yahoo.com

Abstract

Introduction: The Ras/Akt/mTORC1 signal transduction pathways play a critical role in regulating translation and converge on initiation factor eukaryotic translation initiation factor 4E (eIF4E) which is overexpressed in various malignancies. In the current study we aimed to assess the effect of chrysin and caffeic acid phenethyl ester (CAPE) on eIF4E expression level in human stomach cancer AGS cell line.

Methods: AGS cells were treated with 15, 20, 30 and 40 μM concentration of chrysin and CAPE separately, then eIF4E expression was evaluated in treated cells using real time-PCR method.

Results: A significant decrease in eIF4E expression in the cells following 40 μM chrysin treatment was observed (p<0.05). There was a significant decrease in CAPE-treated cells in a dose dependent manner. Indeed the cells treated with 30 and 40 μM concentrations of CAPE, showed a significant decline in eIF4E expression (p<0.05).

Conclusion: Our results suggest that CAPE and chrysin may be useful as a potential therapeutic agent for treatment of gastric cancers with an elevated eIF4E expression level.

Keywords: Gastric cancer, AGS cell line, eIF4E, Chrysin, CAPE

Implication for health policy/practice/research/medical education:

Chrysin and caffeic acid phenethyl ester – two components of propolis decreases eukaryotic translation initiation factor 4E (eIF4E) gene expression and might be useful in developing chemotherapeutic agents for treating human gastric cancer.

Please cite this paper as: Abolhasani M, Hashemzadeh-Chaleshtori M, Doosti A, Amini Sarteshnizi N, Gholami-Arjenaki M, Teimori H. Evaluation of the effect of Chrysin and Caffeic acid phenethyl ester on eIF4E expression in human gastric cancer AGS cell line. J Herbmed Pharmacol. 2014; 3(2): 129-133.

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